Name: Anti-RALA Antibody (CAB0541)
Brand: Cell Cycle Antibodies 1
SKU: CAB0541
Availability: OutOfStock

Antibody Name:	Anti-RALA Antibody
Antibody SKU:	CAB0541
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-206 of human RALA (NP_005393.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	Mouse lung, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-206 of human RALA (NP_005393.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAN KPKG QNSL ALHK VIMV GSGG VGKS ALTL QFMY DEFV EDYE PTKA DSYR KKVV LDGE EVQI DILD TAGQ EDYA AIRD NYFR SGEG FLCV FSIT EMES FAAT ADFR EQIL RVKE DENV PFLL VGNK SDLE DKRQ VSVE EAKN RAEQ WNVN YVET SAKT RANV DKVF FDLM REIR ARKM EDSK EKNG KKKR KSLA KRIR ERCC IL
Gene ID:	5898
Uniprot:	P11233
Cellular Location:	Cell membrane, Cell surface, Cleavage furrow, Cytoplasmic side, Lipid-anchor, Midbody
Calculated MW:	23kDa
Observed MW:	24kDa

Synonyms:	RALA, RAL
Background:	The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins.

UniProt Protein Function:	RALA: Multifuntional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin- dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells. Interacts with RALBP1 via its effector domain. Interacts with EXOC8 and EXOC2. EXOC2 and EXOC8 have overlapping binding sites and compete for RALA binding. Interacts with Clostridium exoenzyme C3. Interacts with RALGPS1. Interacts with LPAR1 and LPAR2. Interacts with ADRBK1 in response to LPAR1 activation. RALA and ADRBK1 mutually inhibit each other's binding to LPAR1. Activated in an LPA-dependent manner by LPAR1 and in an LPA-independent manner by LPAR2. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; G protein, monomeric, Ras; G protein, monomeric; G protein Chromosomal Location of Human Ortholog: 7p15-p13 Cellular Component: cytoplasmic vesicle membrane; cell surface; focal adhesion; endocytic vesicle; plasma membrane; midbody; cleavage furrow Molecular Function:GTPase activity; protein binding; GDP binding; GTP binding; myosin binding; Edg-2 lysophosphatidic acid receptor binding; ubiquitin protein ligase binding; ATPase binding Biological Process: positive regulation of filopodium formation; regulation of exocytosis; exocytosis; lipid raft localization; nerve growth factor receptor signaling pathway; viral reproduction; metabolic process; Ras protein signal transduction; actin cytoskeleton reorganization; cytokinesis; chemotaxis; signal transduction
NCBI Summary:	The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins. [provided by RefSeq, Jul 2008]
UniProt Code:	P11233
NCBI GenInfo Identifier:	131834
NCBI Gene ID:	5898
NCBI Accession:	P11233.1
UniProt Secondary Accession:	P11233,A4D1W3,
UniProt Related Accession:	P11233
Molecular Weight:	206
NCBI Full Name:	Ras-related protein Ral-A
NCBI Synonym Full Names:	v-ral simian leukemia viral oncogene homolog A (ras related)
NCBI Official Symbol:	RALA
NCBI Official Synonym Symbols:	RAL
NCBI Protein Information:	ras-related protein Ral-A; RAS-like protein A; ras related GTP binding protein A; Ras family small GTP binding protein RALA
UniProt Protein Name:	Ras-related protein Ral-A
Protein Family:	Ras-related protein
UniProt Gene Name:	RALA
UniProt Entry Name:	RALA_HUMAN

Western blot analysis of extracts of various cell lines, using RALA antibody (CAB0541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

Anti-RALA Antibody (CAB11736)

RALA Antibody (PACO11701)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)