Name: Anti-RAG1 Antibody (CAB12646)
Brand: Epigenetics & Nuclear Signaling Antibodies 1
SKU: CAB12646
Availability: OutOfStock

Antibody Name:	Anti-RAG1 Antibody
Antibody SKU:	CAB12646
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human RAG1 (NP_000439.1).

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:3000
Reactivity:	Mouse
Positive Samples:	Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human RAG1 (NP_000439.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAS FPPT LGLS SAPD EIQH PHIK FSEW KFKL FRVR SFEK TPEE AQKE KKDS FEGK PSLE QSPA VLDK ADGQ KPVP TQPL LKAH PKFS KKFH DNEK ARGK AIHQ ANLR HLCR ICGN SFRA DEHN RRYP VHGP VDGK TLGL LRKK EKRA TSWP DLIA KVFR IDVK ADVD SIHP TEFC HNCW SIMH RKFS SAPC EVYF PRNV TMEW HPHT PSCD ICNT ARRG LKRK SLQP NLQL SKKL KTVL DQAR QARQ RKRR AQAR ISSK DVMK KIAN CS
Gene ID:	5896
Uniprot:	P15918
Cellular Location:	Nucleus
Calculated MW:	106kDa/119kDa
Observed MW:	110kDa

Synonyms:	RAG1, RAG-1, RNF74
Background:	The protein encoded by this gene is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases.

UniProt Protein Function:	RAG1: Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T- lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'- hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'- phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination. Mediates polyubiquitination of KPNA1. Homodimer. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Maturing lymphoid cells. Belongs to the RAG1 family.
UniProt Protein Details:	Protein type:DNA-binding; EC 3.1.-.-; EC 6.3.2.-; EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase Chromosomal Location of Human Ortholog: 11p12 Cellular Component: nucleoplasm; nucleus Molecular Function:DNA binding; endonuclease activity; histone binding; metal ion binding; protein binding; protein homodimerization activity; sequence-specific DNA binding; ubiquitin-protein ligase activity; zinc ion binding Biological Process: B cell differentiation; DNA recombination; histone monoubiquitination; immune response; pre-B cell allelic exclusion; protein autoubiquitination; T cell differentiation in the thymus; V(D)J recombination Disease: Alpha/beta T-cell Lymphopenia With Gamma/delta T-cell Expansion, Severe Cytomegalovirus Infection, And Autoimmunity; Combined Cellular And Humoral Immune Defects With Granulomas; Omenn Syndrome; Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-negative, Nk Cell-positive
NCBI Summary:	The protein encoded by this gene is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases. [provided by RefSeq, Jul 2008]
UniProt Code:	P15918
NCBI GenInfo Identifier:	313104166
NCBI Gene ID:	5896
NCBI Accession:	P15918.2
UniProt Secondary Accession:	P15918,Q8IY72, Q8NER2, E9PPC4,
UniProt Related Accession:	P15918
Molecular Weight:	119kDa
NCBI Full Name:	V(D)J recombination-activating protein 1
NCBI Synonym Full Names:	recombination activating 1
NCBI Official Symbol:	RAG1
NCBI Official Synonym Symbols:	RAG-1; RNF74
NCBI Protein Information:	V(D)J recombination-activating protein 1
UniProt Protein Name:	V(D)J recombination-activating protein 1
UniProt Synonym Protein Names:	RING finger protein 74Including the following 2 domains:Endonuclease RAG1 (EC:3.1.-.-)E3 ubiquitin-protein ligase RAG1 (EC:2.3.2.27)Alternative name(s):RING-type E3 ubiquitin transferase RAG1Curated
Protein Family:	Seed allergenic protein
UniProt Gene Name:	RAG1

Western blot analysis of extracts of mouse thymus, using RAG1 antibody (CAB12646) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

ELISA Kits

Human RAG1 ELISA Kit

Human V(D)J recombination-activating protein 1 (RAG1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)