Antibody Name:	Anti-RAE1 Antibody
Antibody SKU:	CAB6713
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-368 of human RAE1 (NP_003601.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Mouse spleen, Mouse thymus, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-368 of human RAE1 (NP_003601.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSLF GTTS GFGT SGTS MFGS ATTD NHNP MKDI EVTS SPDD SIGC LSFS PPTL PGNF LIAG SWAN DVRC WEVQ DSGQ TIPK AQQM HTGP VLDV CWSD DGSK VFTA SCDK TAKM WDLS SNQA IQIA QHDA PVKT IHWI KAPN YSCV MTGS WDKT LKFW DTRS SNPM MVLQ LPER CYCA DVIY PMAV VATA ERGL IVYQ LENQ PSEF RRIE SPLK HQHR CVAI FKDK QNKP TGFA LGSI EGRV AIHY INPP NPAK DNFT FKCH RSNG TNTS APQD IYAV NGIA FHPV HGTL ATVG SDGR FSFW DKDA RTKL KTSE QLDQ PISA CCFN HNGN IFAY ASSY DWSK GHEF YNPQ KKNY IFLR NAAE ELKP RNKK
Gene ID:	8480
Uniprot:	P78406
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	40kDa
Observed MW:	40kDa

Synonyms:

RAE1, MIG14, MRNP41, Mnrp41, dJ481F12.3, dJ800J21.1

Background:

Mutations in the Schizosaccharomyces pombe Rae1 and Saccharomyces cerevisiae Gle2 genes have been shown to result in accumulation of poly(A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. The protein encoded by this gene is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

UniProt Protein Function:	RAE1: Binds mRNA. May function in nucleocytoplasmic transport and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Belongs to the WD repeat rae1 family.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; RNA-binding Chromosomal Location of Human Ortholog: 20q13.31 Cellular Component: nuclear outer membrane; cytoskeleton; cytoplasm; nucleolus; nuclear pore; nuclear envelope; nucleus Molecular Function:RNA binding; microtubule binding Biological Process: mRNA export from nucleus; viral reproduction; hexose transport; cytokine and chemokine mediated signaling pathway; carbohydrate metabolic process; mitotic nuclear envelope disassembly; gene expression; viral transcription; pathogenesis; glucose transport; viral infectious cycle; mitotic cell cycle; transmembrane transport
NCBI Summary:	Mutations in the Schizosaccharomyces pombe Rae1 and Saccharomyces cerevisiae Gle2 genes have been shown to result in accumulation of poly(A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. The protein encoded by this gene is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P78406
NCBI GenInfo Identifier:	3122666
NCBI Gene ID:	8480
NCBI Accession:	P78406.1
UniProt Secondary Accession:	P78406,O15306, Q3SYL7, Q5TCH8, Q6V708, Q9H100, Q9NQM6 A8K882,
UniProt Related Accession:	P78406
Molecular Weight:	40kDa
NCBI Full Name:	mRNA export factor
NCBI Synonym Full Names:	ribonucleic acid export 1
NCBI Official Symbol:	RAE1
NCBI Official Synonym Symbols:	MIG14; MRNP41; Mnrp41; dJ481F12.3; dJ800J21.1
NCBI Protein Information:	mRNA export factor; mRNA export protein; rae1 protein homolog; RAE1 RNA export 1 homolog; migration-inducing gene 14; mRNA-binding protein, 41-kD; mRNA-associated protein MRNP 41; homolog of yeast Rae1 (Bharathi) mRNA-associated protein of 41 kDa (Kraemer)
UniProt Protein Name:	mRNA export factor
UniProt Synonym Protein Names:	Rae1 protein homolog; mRNA-associated protein mrnp 41
Protein Family:	Protein
UniProt Gene Name:	RAE1
UniProt Entry Name:	RAE1L_HUMAN

	Western blot analysis of extracts of various cell lines, using RAE1 antibody (CAB6713) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat brain using RAE1 antibody (CAB6713) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using RAE1 antibody (CAB6713) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF-7 cells using RAE1 antibody (CAB6713). Blue: DAPI for nuclear staining.