Antibody Name:	Anti-RAD9A Antibody
Antibody SKU:	CAB1890
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 162-391 of human RAD9A (NP_004575.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	BT-474, 22Rv1, HepG2, MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 162-391 of human RAD9A (NP_004575.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ALAE VTLG IGRG RRVI LRSY HEEE ADST AKAM VTEM CLGE EDFQ QLQA QEGV AITF CLKE FRGL LSFA ESAN LNLS IHFD APGR PAIF TIKD SLLD GHFV LATL SDTD SHSQ DLGS PERH QPVP QLQA HSTP HPDD FAND DIDS YMIA METT IGNE GSRV LPSI SLSP GPQP PKSP GPHS EEED EAEP STVP GTPP PKKF RSLF FGSI LAPV RSPQ GPSP VLAE DSEG EG
Gene ID:	5883
Uniprot:	Q99638
Cellular Location:	Nucleus
Calculated MW:	42kDa
Observed MW:	60kDa

Synonyms:

RAD9A, RAD9

Background:

This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	Rad9: a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. Possesses a 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. Forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for Rad9.
UniProt Protein Details:	Protein type:EC 3.1.11.2; DNA replication; Deoxyribonuclease; Apoptosis; Cell cycle regulation Chromosomal Location of Human Ortholog: 11q13.1-q13.2 Cellular Component: nucleoplasm; cytoplasm; nucleus Molecular Function:protein binding; enzyme binding; histone deacetylase binding; exodeoxyribonuclease III activity; 3'-5' exonuclease activity; SH3 domain binding; protein kinase binding Biological Process: intra-S DNA damage checkpoint; DNA damage checkpoint; DNA replication checkpoint; DNA replication; DNA repair; DNA catabolic process, exonucleolytic; response to DNA damage stimulus
NCBI Summary:	This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
UniProt Code:	Q99638
NCBI GenInfo Identifier:	74717382
NCBI Gene ID:	5883
NCBI Accession:	Q99638.1
UniProt Related Accession:	Q99638
Molecular Weight:
NCBI Full Name:	Cell cycle checkpoint control protein RAD9A
NCBI Synonym Full Names:	RAD9 checkpoint clamp component A
NCBI Official Symbol:	RAD9A
NCBI Official Synonym Symbols:	RAD9
NCBI Protein Information:	cell cycle checkpoint control protein RAD9A
UniProt Protein Name:	Cell cycle checkpoint control protein RAD9A
UniProt Synonym Protein Names:	DNA repair exonuclease rad9 homolog A
Protein Family:	RAD9, HUS1, RAD1-interacting nuclear orphan protein
UniProt Gene Name:	RAD9A
UniProt Entry Name:	RAD9A_HUMAN

	Western blot analysis of extracts of various cell lines, using RAD9A antibody (CAB1890) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
	Immunohistochemistry of paraffin-embedded rat liver using RAD9A antibody (CAB1890) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver damage using RAD9A antibody (CAB1890) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of MCF-7 cells using RAD9A antibody (CAB1890). Blue: DAPI for nuclear staining.