Epigenetics & Nuclear Signaling Antibodies 2

Anti-RAD54B Antibody (CAB16208)

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SKU:
CAB16208
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-RAD54B Antibody
Antibody SKU:CAB16208
Antibody Size:20uL, 50uL, 100uL
Application:WB IF IP
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 781-910 of human RAD54B (NP_036547.1).
Application:WB IF IP
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:HeLa, Jurkat, DU145, HT-29, mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 781-910 of human RAD54B (NP_036547.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RQIS KQGL CGAV VDLT KTSE HIQF SVEE LKNL FTLH ESSD CVTH DLLD CECT GEEV HTGD SLEK FIVS RDCQ LGPH HQKS NSLK PLSM SQLK QWKH FSGD HLNL TDPF LERI TENV SFIF QNIT TQAT GT
Gene ID:25788
Uniprot:Q9Y620
Cellular Location:
Calculated MW:103kDa
Observed MW:103kDa
Synonyms:RAD54B, RDH54
Background:The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer.
UniProt Protein Function:Involved in DNA repair and mitotic recombination. May play an active role in recombination processes in concert with other members of the RAD52 epistasis group.
NCBI Summary:The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer. [provided by RefSeq, Jul 2008]
UniProt Code:Q9Y620
NCBI GenInfo Identifier:51316548
NCBI Gene ID:25788
NCBI Accession:Q9Y620.1
UniProt Secondary Accession:Q9Y620,F6WBS8,
UniProt Related Accession:Q9Y620
Molecular Weight:13,330 Da
NCBI Full Name:DNA repair and recombination protein RAD54B
NCBI Synonym Full Names:RAD54 homolog B (S. cerevisiae)
NCBI Official Symbol:RAD54B  
NCBI Official Synonym Symbols:RDH54  
NCBI Protein Information:DNA repair and recombination protein RAD54B
UniProt Protein Name:DNA repair and recombination protein RAD54B
UniProt Synonym Protein Names:RAD54 homolog B
Protein Family:DNA repair and recombination protein
UniProt Gene Name:RAD54B  
UniProt Entry Name:RA54B_HUMAN
Anti-RAD54B Antibody (CAB16208)Western blot analysis of extracts of various cell lines, using RAD54B antibody (CAB16208) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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