Epigenetics & Nuclear Signaling Antibodies 3
Anti-Rad52 Antibody (CAB3077)
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- SKU:
- CAB3077
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-Rad52 Antibody |
| Antibody SKU: | CAB3077 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 125-418 of human Rad52 (NP_602296.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:1000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HL-60, Mouse thymus, Rat thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 125-418 of human Rad52 (NP_602296.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GYGV SEGL KSKA LSLE KARK EAVT DGLK RALR SFGN ALGN CILD KDYL RSLN KLPR QLPL EVDL TKAK RQDL EPSV EEAR YNSC RPNM ALGH PQLQ QVTS PSRP SHAV IPAD QDCS SRSL SSSA VESE ATHQ RKLR QKQL QQQF RERM EKQQ VRVS TPSA EKSE AAPP APPV THST PVTV SEPL LEKD FLAG VTQE LIKT LEDN SEKW AVTP DAGD GVVK PSSR ADPA QTSD TLAL NNQM VTQN RTPH SVCH QKPQ AKSG SWDL QTYS ADQR TTGN WESH RKSQ DMKK RKYD PS |
| Gene ID: | 5893 |
| Uniprot: | P43351 |
| Cellular Location: | Nucleus |
| Calculated MW: | 12kDa/15kDa/24kDa/46kDa |
| Observed MW: | 46kDa |
| Synonyms: | RAD52 |
| Background: | The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. |
| UniProt Protein Function: | RAD52: a protein involved in DNA double strand breaks and recombination. Associates with and is phosphorylated by c-Abl. Belongs to the RAD52/RAD22 family. |
| UniProt Protein Details: | Protein type:DNA repair, damage Chromosomal Location of Human Ortholog: 12p13-p12.2 Cellular Component: nucleoplasm; nucleus; protein complex Molecular Function:DNA binding; identical protein binding; protein binding Biological Process: DNA recombination; double-strand break repair; protein sumoylation; response to DNA damage stimulus |
| NCBI Summary: | The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014] |
| UniProt Code: | P43351 |
| NCBI GenInfo Identifier: | 1172823 |
| NCBI Gene ID: | 5893 |
| NCBI Accession: | P43351.1 |
| UniProt Secondary Accession: | P43351,Q13205, Q9Y5T7, Q9Y5T8, Q9Y5T9, |
| UniProt Related Accession: | P43351 |
| Molecular Weight: | 12,943 Da |
| NCBI Full Name: | DNA repair protein RAD52 homolog |
| NCBI Synonym Full Names: | RAD52 homolog, DNA repair protein |
| NCBI Official Symbol: | RAD52 |
| NCBI Protein Information: | DNA repair protein RAD52 homolog |
| UniProt Protein Name: | DNA repair protein RAD52 homolog |
| Protein Family: | RAD52 motif-containing protein |
| UniProt Gene Name: | RAD52 |
| UniProt Entry Name: | RAD52_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Rad52 antibody (CAB3077) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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