Epigenetics & Nuclear Signaling Antibodies 2
Anti-Rad51 Antibody (CAB6268)
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- SKU:
- CAB6268
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-Rad51 Antibody |
| Antibody SKU: | CAB6268 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Rad51 (NP_002866.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:1000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-937, Mouse brain, Mouse ovary, Mouse testis, Rat testis |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Rad51 (NP_002866.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EAVA YAPK KELI NIKG ISEA KADK ILAE AAKL VPMG FTTA TEFH QRRS EIIQ ITTG SKEL DKLL QGGI ETGS ITEM FGEF RTGK TQIC HTLA VTCQ LPID R |
| Gene ID: | 5888 |
| Uniprot: | Q06609 |
| Cellular Location: | Cytoplasm, Mitochondrion matrix, Nucleus, centrosome, cytoskeleton, microtubule organizing center, perinuclear region |
| Calculated MW: | 26kDa/31kDa/36kDa |
| Observed MW: | 37kDa |
| Synonyms: | RAD51, BRCC5, FANCR, HRAD51, HsRad51, HsT16930, MRMV2, RAD51A, RECA |
| Background: | The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR). Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair (PubMed:26253028). |
| NCBI Summary: | The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009] |
| UniProt Code: | Q06609 |
| NCBI GenInfo Identifier: | 548663 |
| NCBI Gene ID: | 5888 |
| NCBI Accession: | Q06609.1 |
| UniProt Secondary Accession: | Q06609,Q6FHX9, Q6ZNA8, Q9BV60, B0FXP0, B2R8T6, |
| UniProt Related Accession: | Q06609 |
| Molecular Weight: | |
| NCBI Full Name: | DNA repair protein RAD51 homolog 1 |
| NCBI Synonym Full Names: | RAD51 recombinase |
| NCBI Official Symbol: | RAD51 |
| NCBI Official Synonym Symbols: | RECA; BRCC5; FANCR; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930 |
| NCBI Protein Information: | DNA repair protein RAD51 homolog 1 |
| UniProt Protein Name: | DNA repair protein RAD51 homolog 1 |
| UniProt Synonym Protein Names: | RAD51 homolog A |
| Protein Family: | DNA repair protein |
| UniProt Gene Name: | RAD51 |
| UniProt Entry Name: | RAD51_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Rad51 antibody (CAB6268) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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