Name: Anti-Rad21 Antibody (CAB18850)
SKU: CAB18850
Availability: OutOfStock

Product Name:	Rad21 Rabbit pAb
Product Code:	CAB18850
Size:	20uL, 50uL, 100uL
Synonyms:	CDLS4, HR21, HRAD21, MCD1, NXP1, SCC1, hHR21
Applications:	WB, IHC, IF, IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human Rad21 .

Applications:	WB, IHC, IF, IP
Recommended Dilutions:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, Mouse thymus, C6

Immunogen:	Recombinant protein of human Rad21 .
Purification Method:	Affinity purification
Storage:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	5885
Uniprot:	O60216
Observed MW:	130KDa

UniProt Protein Function:	RAD21: Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway. Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. Found in a complex with SMC1A, SMC3, CDCA5, PDS5A/APRIN and PDS5B/SCC-112. Interacts with PDS5B and WAPAL; the interaction is direct. Belongs to the rad21 family.Chromosomal Location of Human Ortholog: 8q24Cellular Component: chromatin; chromosome; chromosome, pericentric region; cohesin complex; cytosol; membrane; nucleoplasmMolecular Function: protein bindingBiological Process: apoptosis; cell division; cellular protein metabolic process; chromosome segregation; DNA recombination; double-strand break repair; meiotic recombination; mitosis; mitotic cell cycle; positive regulation of transcription from RNA polymerase II promoter; post-translational protein modification; protein sumoylation; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoterDisease: Cornelia De Lange Syndrome 4
UniProt Protein Details:
NCBI Summary:	The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008]
UniProt Code:	O60216
NCBI GenInfo Identifier:	25091097
NCBI Gene ID:	5885
NCBI Accession:	O60216.2
UniProt Secondary Accession:	O60216,Q15001, Q99568, A8K0E0
UniProt Related Accession:	O60216
Molecular Weight:	71,690 Da
NCBI Full Name:	Double-strand-break repair protein rad21 homolog
NCBI Synonym Full Names:	RAD21 cohesin complex component
NCBI Official Symbol:	RAD21
NCBI Official Synonym Symbols:	HR21; MCD1; NXP1; SCC1; CDLS4; hHR21; HRAD21
NCBI Protein Information:	double-strand-break repair protein rad21 homolog
UniProt Protein Name:	Double-strand-break repair protein rad21 homolog
UniProt Synonym Protein Names:	Nuclear matrix protein 1; NXP-1; SCC1 homolog
Protein Family:	Cofilin
UniProt Gene Name:	RAD21
UniProt Entry Name:	RAD21_HUMAN

	Western blot analysis of extracts of various cell lines, using Rad21 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
	Western blot analysis of extracts of C6 cells, using Rad21 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using Rad21 Rabbit pAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded mouse stomach using Rad21 Rabbit pAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded rat stomach using Rad21 Rabbit pAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Immunofluorescence analysis of U2OS cells using Rad21 Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Rad21 antibody at a dilition of 1:1000.
	Chromatin immunoprecipitation analysis of extracts of A-549 cells, using Rad21 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.