Epigenetics & Nuclear Signaling Antibodies 1
Anti-RAD1 Antibody (CAB1047)
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- SKU:
- CAB1047
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-RAD1 Antibody |
| Antibody SKU: | CAB1047 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human RAD1 (NP_002844.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, 293T, SH-SY5Y, Mouse spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human RAD1 (NP_002844.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPLL TQQI QDED DQYS LVAS LDNV RNLS TILK AIHF REHA TCFA TKNG IKVT VENA KCVQ ANAF IQAG IFQE FKVQ EESV TFRI NLTV LLDC LSIF GSSP MPGT LTAL RMCY QGYG YPLM LFLE EGGV VTVC KINT QEPE ETLD FDFC STNV INKI ILQS EGLR EAFS ELDM TSEV LQIT MSPD KPYF RLST FGNA GSSH LDYP KDSD LMEA FHCN QTQV NRYK ISLL KPST KALV LSCK VSIR TDNR GFLS LQYM IRNE DGQI CFVE YYCC PDEE VPES ES |
| Gene ID: | 5810 |
| Uniprot: | O60671 |
| Cellular Location: | Nucleus |
| Calculated MW: | 7kDa/27kDa/31kDa |
| Observed MW: | 32kDa |
| Synonyms: | RAD1, HRAD1, REC1 |
| Background: | This gene encodes a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. The 9-1-1 complex is recruited by RAD17 to affected sites where it may attract specialized DNA polymerases and other DNA repair effectors. Alternatively spliced transcript variants of this gene have been described. |
| UniProt Protein Function: | RAD1: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity. Belongs to the rad1 family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; DNA repair, damage; Deoxyribonuclease; EC 3.1.11.2 Chromosomal Location of Human Ortholog: 5p13.2 Cellular Component: nucleoplasm; intracellular membrane-bound organelle; chromosome; nucleus Molecular Function:protein binding; exodeoxyribonuclease III activity; damaged DNA binding; 3'-5' exonuclease activity Biological Process: meiotic recombination checkpoint; substantia nigra development; DNA damage checkpoint; DNA repair; DNA catabolic process, exonucleolytic; DNA replication; response to DNA damage stimulus |
| NCBI Summary: | This gene encodes a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. The 9-1-1 complex is recruited by RAD17 to affected sites where it may attract specialized DNA polymerases and other DNA repair effectors. Alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Jan 2009] |
| UniProt Code: | O60671 |
| NCBI GenInfo Identifier: | 74735450 |
| NCBI Gene ID: | 5810 |
| NCBI Accession: | O60671.1 |
| UniProt Secondary Accession: | O60671,O75572, O95304, Q1W161, Q5KSM0, Q5KSM1, Q9UEP1 |
| UniProt Related Accession: | O60671 |
| Molecular Weight: | 31,827 Da |
| NCBI Full Name: | Cell cycle checkpoint protein RAD1 |
| NCBI Synonym Full Names: | RAD1 checkpoint DNA exonuclease |
| NCBI Official Symbol: | RAD1 |
| NCBI Official Synonym Symbols: | REC1; HRAD1 |
| NCBI Protein Information: | cell cycle checkpoint protein RAD1; RAD1 homolog; exonuclease homolog RAD1; DNA repair exonuclease REC1; checkpoint control protein HRAD1; DNA repair exonuclease rad1 homolog; cell cycle checkpoint protein Hrad1; rad1-like DNA damage checkpoint protein |
| UniProt Protein Name: | Cell cycle checkpoint protein RAD1 |
| UniProt Synonym Protein Names: | DNA repair exonuclease rad1 homolog; Rad1-like DNA damage checkpoint protein |
| Protein Family: | DNA repair protein |
| UniProt Gene Name: | RAD1 |
| UniProt Entry Name: | RAD1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RAD1 antibody (CAB1047) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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