Cell Cycle Antibodies 1
Anti-RACGAP1 Antibody (CAB5298)
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- SKU:
- CAB5298
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-RACGAP1 Antibody |
| Antibody SKU: | CAB5298 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 353-632 of human RACGAP1 (NP_001119576.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | HeLa, K-562, Jurkat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 353-632 of human RACGAP1 (NP_001119576.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DFVS QTSP MIPS IVVH CVNE IEQR GLTE TGLY RISG CDRT VKEL KEKF LRVK TVPL LSKV DDIH AICS LLKD FLRN LKEP LLTF RLNR AFME AAEI TDED NSIA AMYQ AVGE LPQA NRDT LAFL MIHL QRVA QSPH TKMD VANL AKVF GPTI VAHA VPNP DPVT MLQD IKRQ PKVV ERLL SLPL EYWS QFMM VEQE NIDP LHVI ENSN AFST PQTP DIKV SLLG PVTT PEHQ LLKT PSSS SLSQ RVRS TLTK NTPR FGSK SKSA TNLG RQGN FFAS PMLK |
| Gene ID: | 29127 |
| Uniprot: | Q9H0H5 |
| Cellular Location: | Cell membrane, Cleavage furrow, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Midbody, Nucleus, Peripheral membrane protein, acrosome, cytoskeleton, secretory vesicle, spindle |
| Calculated MW: | 71kDa |
| Observed MW: | 82kDa |
| Synonyms: | RACGAP1, CYK4, HsCYK-4, ID-GAP, MgcRacGAP |
| Background: | This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12. |
| UniProt Protein Function: | MgcRacGAP: a rac GTPase activating protein. Plays an important role in cytokinesis by regulating cortical movement through RhoA. Phosphorylation by Aurora B apparently induces GAP activity toward RhoA. |
| UniProt Protein Details: | Protein type:GAPs; GAPs, Rac/Rho Chromosomal Location of Human Ortholog: 12q13.12 Cellular Component: nucleoplasm; microtubule; extrinsic to internal side of plasma membrane; acrosome; spindle midzone; midbody; nucleus; cytosol; cleavage furrow Molecular Function:gamma-tubulin binding; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; metal ion binding; microtubule binding; beta-tubulin binding; protein kinase binding; GTPase activator activity; alpha-tubulin binding Biological Process: positive regulation of cytokinesis; regulation of attachment of spindle microtubules to kinetochore; spindle midzone assembly involved in mitosis; antigen processing and presentation of exogenous peptide antigen via MHC class II; sulfate transport; microtubule-based movement; regulation of small GTPase mediated signal transduction; embryonic development; cytokinesis after mitosis; small GTPase mediated signal transduction; neuroblast proliferation; spermatogenesis; blood coagulation; cytokinesis, contractile ring formation; positive regulation of GTPase activity |
| NCBI Summary: | The protein encoded by this gene belongs to the GTPase-activating protein (GAP) family. GAPs bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, GAPs negatively regulate Rho-mediated signals. This protein plays a regulatory role in initiation of cytokinesis, controlling cell growth and differentiation of hematopoietic cells, regulating spermatogenesis, and in neuronal proliferation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2011] |
| UniProt Code: | Q9H0H5 |
| NCBI GenInfo Identifier: | 74762727 |
| NCBI Gene ID: | 29127 |
| NCBI Accession: | Q9H0H5.1 |
| UniProt Secondary Accession: | Q9H0H5,Q6PJ26, Q9NWN2, Q9P250, Q9P2W2, |
| UniProt Related Accession: | Q9H0H5 |
| Molecular Weight: | 632 |
| NCBI Full Name: | Rac GTPase-activating protein 1 |
| NCBI Synonym Full Names: | Rac GTPase activating protein 1 |
| NCBI Official Symbol: | RACGAP1 |
| NCBI Official Synonym Symbols: | CYK4; ID-GAP; HsCYK-4; MgcRacGAP |
| NCBI Protein Information: | rac GTPase-activating protein 1; RACGAP1; protein CYK4 homolg; protein CYK4 homolog; male germ cell RacGap; GTPase activating protein |
| UniProt Protein Name: | Rac GTPase-activating protein 1 |
| UniProt Synonym Protein Names: | Male germ cell RacGap; MgcRacGAP; Protein CYK4 homolog; CYK4; HsCYK-4 |
| Protein Family: | Rac GTPase-activating protein |
| UniProt Gene Name: | RACGAP1 |
| UniProt Entry Name: | RGAP1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RACGAP1 antibody (CAB5298) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using RACGAP1 antibody (CAB5298). Blue: DAPI for nuclear staining. |
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