Cell Cycle Antibodies 1

Anti-RACGAP1 Antibody (CAB5298)

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SKU:
CAB5298
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-RACGAP1 Antibody
Antibody SKU:CAB5298
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 353-632 of human RACGAP1 (NP_001119576.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human
Positive Samples:HeLa, K-562, Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 353-632 of human RACGAP1 (NP_001119576.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DFVS QTSP MIPS IVVH CVNE IEQR GLTE TGLY RISG CDRT VKEL KEKF LRVK TVPL LSKV DDIH AICS LLKD FLRN LKEP LLTF RLNR AFME AAEI TDED NSIA AMYQ AVGE LPQA NRDT LAFL MIHL QRVA QSPH TKMD VANL AKVF GPTI VAHA VPNP DPVT MLQD IKRQ PKVV ERLL SLPL EYWS QFMM VEQE NIDP LHVI ENSN AFST PQTP DIKV SLLG PVTT PEHQ LLKT PSSS SLSQ RVRS TLTK NTPR FGSK SKSA TNLG RQGN FFAS PMLK
Gene ID:29127
Uniprot:Q9H0H5
Cellular Location:Cell membrane, Cleavage furrow, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Midbody, Nucleus, Peripheral membrane protein, acrosome, cytoskeleton, secretory vesicle, spindle
Calculated MW:71kDa
Observed MW:82kDa
Synonyms:RACGAP1, CYK4, HsCYK-4, ID-GAP, MgcRacGAP
Background:This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12.
UniProt Protein Function:MgcRacGAP: a rac GTPase activating protein. Plays an important role in cytokinesis by regulating cortical movement through RhoA. Phosphorylation by Aurora B apparently induces GAP activity toward RhoA.
UniProt Protein Details:

Protein type:GAPs; GAPs, Rac/Rho

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: nucleoplasm; microtubule; extrinsic to internal side of plasma membrane; acrosome; spindle midzone; midbody; nucleus; cytosol; cleavage furrow

Molecular Function:gamma-tubulin binding; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; metal ion binding; microtubule binding; beta-tubulin binding; protein kinase binding; GTPase activator activity; alpha-tubulin binding

Biological Process: positive regulation of cytokinesis; regulation of attachment of spindle microtubules to kinetochore; spindle midzone assembly involved in mitosis; antigen processing and presentation of exogenous peptide antigen via MHC class II; sulfate transport; microtubule-based movement; regulation of small GTPase mediated signal transduction; embryonic development; cytokinesis after mitosis; small GTPase mediated signal transduction; neuroblast proliferation; spermatogenesis; blood coagulation; cytokinesis, contractile ring formation; positive regulation of GTPase activity

NCBI Summary:The protein encoded by this gene belongs to the GTPase-activating protein (GAP) family. GAPs bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, GAPs negatively regulate Rho-mediated signals. This protein plays a regulatory role in initiation of cytokinesis, controlling cell growth and differentiation of hematopoietic cells, regulating spermatogenesis, and in neuronal proliferation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt Code:Q9H0H5
NCBI GenInfo Identifier:74762727
NCBI Gene ID:29127
NCBI Accession:Q9H0H5.1
UniProt Secondary Accession:Q9H0H5,Q6PJ26, Q9NWN2, Q9P250, Q9P2W2,
UniProt Related Accession:Q9H0H5
Molecular Weight:632
NCBI Full Name:Rac GTPase-activating protein 1
NCBI Synonym Full Names:Rac GTPase activating protein 1
NCBI Official Symbol:RACGAP1  
NCBI Official Synonym Symbols:CYK4; ID-GAP; HsCYK-4; MgcRacGAP  
NCBI Protein Information:rac GTPase-activating protein 1; RACGAP1; protein CYK4 homolg; protein CYK4 homolog; male germ cell RacGap; GTPase activating protein
UniProt Protein Name:Rac GTPase-activating protein 1
UniProt Synonym Protein Names:Male germ cell RacGap; MgcRacGAP; Protein CYK4 homolog; CYK4; HsCYK-4
Protein Family:Rac GTPase-activating protein
UniProt Gene Name:RACGAP1  
UniProt Entry Name:RGAP1_HUMAN
Anti-RACGAP1 Antibody (CAB5298)Western blot analysis of extracts of various cell lines, using RACGAP1 antibody (CAB5298) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-RACGAP1 Antibody (CAB5298)Immunofluorescence analysis of U2OS cells using RACGAP1 antibody (CAB5298). Blue: DAPI for nuclear staining.
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