Cell Biology Antibodies 5

Anti-RABIF Antibody (CAB15090)

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SKU:
CAB15090
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-RABIF Antibody
Antibody SKU:CAB15090
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-123 of human RABIF (NP_002862.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:DU145, mouse testis, rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-123 of human RABIF (NP_002862.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEPA EQPS ELVS AEGR NRKA VLCQ RCGS RVLQ PGTA LFSR RQLF LPSM RKKP ALSD GSNP DGDL LQEH WLVE DMFI FENV GFTK DVGN IKFL VCAD CEIG PIGW HCLD DKNS FYVA LERV SHE
Gene ID:5877
Uniprot:P47224
Cellular Location:
Calculated MW:13kDa
Observed MW:14kDa
Synonyms:RABIF, MSS4, RASGFR3, RASGRF3
Background:This gene encodes a member of the SCE4/YPT1/RAB family of small GTP-binding proteins that are involved in the regulation of intracellular vesicular transport. This protein stimulates GTP-GDP exchange in SEC4, and to a lesser extent in YPT1 and RAB3A, and may play a general role in vesicular transport.
UniProt Protein Function:RasGRF3: Guanine-nucleotide-releasing protein that acts on members of the SCE4/YPT1/RAB subfamily. Stimulates GDP release from both YPT1 and RAB3A, but is less active on these proteins than on the SEC4 protein. Might play a general role in vesicular transport. Belongs to the DSS4/MSS4 family.
UniProt Protein Details:

Protein type:GAPs; GAPs, Rab

Chromosomal Location of Human Ortholog: 1q32.1

Molecular Function:protein binding; zinc ion binding; guanyl-nucleotide exchange factor activity

Biological Process: protein transport; small GTPase mediated signal transduction; positive regulation of GTPase activity

NCBI Summary:This gene encodes a member of the SCE4/YPT1/RAB family of small GTP-binding proteins that are involved in the regulation of intracellular vesicular transport. This protein stimulates GTP-GDP exchange in SEC4, and to a lesser extent in YPT1 and RAB3A, and may play a general role in vesicular transport. [provided by RefSeq, Oct 2011]
UniProt Code:P47224
NCBI GenInfo Identifier:19923149
NCBI Gene ID:5877
NCBI Accession:NP_002862.2
UniProt Related Accession:P47224
Molecular Weight:
NCBI Full Name:guanine nucleotide exchange factor MSS4
NCBI Synonym Full Names:RAB interacting factor
NCBI Official Symbol:RABIF  
NCBI Official Synonym Symbols:MSS4; RASGFR3; RASGRF3  
NCBI Protein Information:guanine nucleotide exchange factor MSS4
UniProt Protein Name:Guanine nucleotide exchange factor MSS4
UniProt Synonym Protein Names:Rab-interacting factor
Protein Family:Probable phosphatidylinositol 4-phosphate 5-kinase
UniProt Gene Name:RABIF  
UniProt Entry Name:MSS4_HUMAN
Anti-RABIF Antibody (CAB15090)Western blot analysis of extracts of various cell lines, using RABIF antibody (CAB15090) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-RABIF Antibody (CAB15090)Immunofluorescence analysis of L929 cells using RABIF Polyclonal Antibody (CAB15090) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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