Protein Tags
Anti-Rabbit anti Myc-Tag Polyclonal Antibody-C-terminal (CABE009)
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- SKU:
- CABE009
- Product Type:
- Antibody
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
Description
| Antibody Name: | Anti-Rabbit anti Myc-Tag Polyclonal Antibody-C-terminal |
| Antibody SKU: | CABE009 |
| Antibody Size: | 100 uL |
| Application: | WB IF IP |
| Reactivity: | |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to Myc tag. |
| Application: | WB IF IP |
| Recommended Dilution: | WB 1:1000 - 1:10000 IF 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | |
| Positive Samples: | 293T |
| Immunogen: | A synthetic peptide corresponding to Myc tag. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EQKL ISEE DL |
| Gene ID: | |
| Uniprot: | |
| Cellular Location: | |
| Calculated MW: | |
| Observed MW: | Refer to Figures |
 | Western blot analysis of extracts of normal 293T cells and 293T transfected with Myc-NLK protein, using Myc-Tag antibody (CABE009) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of HeLa-Myc-CandHeLa cells using Rabbit anti Myc-Tag pAb-C-terminal (CABE009) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug Rabbit anti Myc-Tag pAb-C-terminal antibody (CABE009). Western blot was performed from the immunoprecipitate using Rabbit anti Myc-Tag pAb-C-terminal antibody (CABE009) at a dilition of 1:50000. |
| Synonyms: | Myc, Myc tag, Myc-tag |
| Background: | Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification. |
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