Protein Tags
Anti-Rabbit anti GFP-Tag Polyclonal Antibody (CABE011)
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- SKU:
- CABE011
- Product Type:
- Antibody
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
Description
| Antibody Name: | Anti-Rabbit anti GFP-Tag Polyclonal Antibody |
| Antibody SKU: | CABE011 |
| Antibody Size: | 100 uL |
| Application: | WB IHC IF |
| Reactivity: | |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein containing a sequence corresponding to amino acids 1-238 of GFP protein. |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:2000 - 1:3000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | |
| Positive Samples: | 293T |
| Immunogen: | Recombinant protein containing a sequence corresponding to amino acids 1-238 of GFP protein. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSKG EELF TGVV PILV ELDG DVNG HKFS VSGE GEGD ATYG KLTL KFIC TTGK LPVP WPTL VTTF SYGV QCFS RYPD HMKQ HDFF KSAM PEGY VQER TIFF KDDG NYKT RAEV KFEG DTLV NRIE LKGI DFKE DGNI LGHK LEYN YNSH NVYI MADK QKNG IKVN FKIR HNIE DGSV QLAD HYQQ NTPI GDGP VLLP DNHY LSTQ SALS KDPN EKRD HMVL LEFV TAAG ITHG MDEL YK |
| Gene ID: | |
| Uniprot: | P42212 |
| Cellular Location: | |
| Calculated MW: | 27kDa |
| Observed MW: | Refer to Figures |
 | Western blot analysis of extracts of normal 293T cells and 293T transfected with GFP-tagged fusion protein, using GFP antibody (CABE011) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of GFP transgenic 293T cells using GFP-Tag antibody (CABE011). Green: GFP expression. Blue: DAPI for nuclear staining.Ã |
| Synonyms: | GFP, GFP tag, GFP-tag |
| Background: | The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. It has been used in modified forms to make biosensors, and many animals have been created that express GFP, which demonstrates a proof of concept that a gene can be expressed throughout a given organism, in selected organs, or in cells of interest. GFP can be introduced into animals or other species through transgenic techniques, and maintained in their genome and that of their offspring. To date, GFP has been expressed in many species, including bacteria, yeasts, fungi, fish and mammals, including in human cells. Scientists Roger Y. Tsien, Osamu Shimomura, and Martin Chalfie were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein. |
| UniProt Protein Function: | Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin. |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | P42212 |
| NCBI GenInfo Identifier: | 1169893 |
| NCBI Gene ID: | |
| NCBI Accession: | P42212.1 |
| UniProt Secondary Accession: | P42212,Q17104, Q27903, Q93125 |
| UniProt Related Accession: | P42212 |
| Molecular Weight: | |
| NCBI Full Name: | Green fluorescent protein |
| NCBI Synonym Full Names: | |
| NCBI Official Symbol: | |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | |
| UniProt Protein Name: | Green fluorescent protein |
| UniProt Synonym Protein Names: | |
| Protein Family: | Green fluorescent protein |
| UniProt Gene Name: | GFP |
| UniProt Entry Name: | GFP_AEQVI |
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