Cell Biology Antibodies 11
Anti-RAB5B Antibody (CAB7447)
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- SKU:
- CAB7447
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-RAB5B Antibody |
| Antibody SKU: | CAB7447 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human RAB5B (NP_002859.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, SKOV3, Mouse ovary, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human RAB5B (NP_002859.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MTSR STAR PNGQ PQAS KICQ FKLV LLGE SAVG KSSL VLRF VKGQ FHEY QEST IGAA FLTQ SVCL DDTT VKFE IWDT AGQE RYHS LAPM YYRG AQAA IVVY DITN QETF ARAK TWVK ELQR QASP SIVI ALAG NKAD LANK RMVE YEEA QAYA DDNS LLFM ETSA KTAM NVND LFLA IAKK LPKS EPQN LGGA AGRS RGVD LHEQ SQQN KSQC CSN |
| Gene ID: | 5869 |
| Uniprot: | P61020 |
| Cellular Location: | Cell membrane, Cytoplasmic side, Early endosome membrane, Lipid-anchor, Melanosome |
| Calculated MW: | 19kDa/23kDa |
| Observed MW: | 24kDa |
| Synonyms: | RAB5B |
| Background: |
| UniProt Protein Function: | RAB5B: Protein transport. Probably involved in vesicular traffic. Belongs to the small GTPase superfamily. Rab family. |
| UniProt Protein Details: | Protein type:G protein; G protein, monomeric, Rab; G protein, monomeric Chromosomal Location of Human Ortholog: 12q13 Cellular Component: membrane; endocytic vesicle; early endosome membrane; early endosome; plasma membrane; melanosome; intracellular; endosome Molecular Function:GTPase activity; protein binding; GDP binding; GTP binding; GTP-dependent protein binding Biological Process: antigen processing and presentation; intracellular protein transport; endosome organization and biogenesis; metabolic process; regulation of endocytosis; plasma membrane to endosome transport; Rab protein signal transduction |
| UniProt Code: | P61020 |
| NCBI GenInfo Identifier: | 46577637 |
| NCBI Gene ID: | 5869 |
| NCBI Accession: | P61020.1 |
| UniProt Related Accession: | P61020 |
| Molecular Weight: | |
| NCBI Full Name: | Ras-related protein Rab-5B |
| NCBI Synonym Full Names: | RAB5B, member RAS oncogene family |
| NCBI Official Symbol: | RAB5B |
| NCBI Protein Information: | ras-related protein Rab-5B |
| UniProt Protein Name: | Ras-related protein Rab-5B |
| Protein Family: | Ras-related protein |
| UniProt Gene Name: | RAB5B |
| UniProt Entry Name: | RAB5B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RAB5B antibody (CAB7447) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
 | Immunofluorescence analysis of L929 cells using RAB5B antibody (CAB7447) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of human colon carcinoma using RAB5B antibody (CAB7447) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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