Name: Anti-RAB4A Antibody (CAB6712)
Brand: Cell Biology Antibodies 10
SKU: CAB6712
Availability: OutOfStock

Antibody Name:	Anti-RAB4A Antibody
Antibody SKU:	CAB6712
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human RAB4A (NP_004569.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	22Rv1, NCI-H460, SKOV3, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human RAB4A (NP_004569.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSQT AMSE TYDF LFKF LVIG NAGT GKSC LLHQ FIEK KFKD DSNH TIGV EFGS KIIN VGGK YVKL QIWD TAGQ ERFR SVTR SYYR GAAG ALLV YDIT SRET YNAL TNWL TDAR MLAS QNIV IILC GNKK DLDA DREV TFLE ASRF AQEN ELMF LETS ALTG ENVE EAFV QCAR KILN KIES GELD PERM GSGI QYGD AALR QLRS PRRA QAPN AQEC GC
Gene ID:	5867
Uniprot:	P20338
Cellular Location:	Cytoplasm, Membrane, Peripheral membrane protein
Calculated MW:	24kDa
Observed MW:	24kDa

Synonyms:

RAB4A, HRES-1, HRES-1/RAB4, HRES1, RAB4

Background:

This gene is a member of the largest group in the Ras superfamily of small GTPases, which regulate membrane trafficking. The encoded protein is associated with early endosomes and is involved in their sorting and recycling. The protein also plays a role in regulating the recycling of receptors from endosomes to the plasma membrane. Alternatively spliced transcript variants have been observed for this gene.

UniProt Protein Function:	Rab4: a member of the RAB family of RAS-related GTP-binding proteins, important regulators of vesicular transport and are located in specific intracellular compartments. RAB7 has been localized to late endosomes and shown to be important in the late endocytic pathway. In addition, it has been shown to have a fundamental role in the cellular vacuolation induced by the cytotoxin VacA of Helicobacter pylori. Mutations in this protein cause Charcot-Marie-Tooth type 2B neuropathy.
UniProt Protein Details:	Protein type:G protein, monomeric; G protein, monomeric, Rab; G protein Chromosomal Location of Human Ortholog: 1q42-q43 Cellular Component: membrane; perinuclear region of cytoplasm; vesicle; endosome Molecular Function:GTPase activity; protein binding; GDP binding; GTP binding; protein transporter activity Biological Process: antigen processing and presentation; intracellular protein transport; metabolic process; regulation of endocytosis; Rab protein signal transduction
NCBI Summary:	This gene is a member of the largest group in the Ras superfamily of small GTPases, which regulate membrane trafficking. The encoded protein is associated with early endosomes and is involved in their sorting and recycling. The protein also plays a role in regulating the recycling of receptors from endosomes to the plasma membrane. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2012]
UniProt Code:	P20338
NCBI GenInfo Identifier:	460018296
NCBI Gene ID:	5867
NCBI Accession:	P20338.3
UniProt Secondary Accession:	P20338,Q5T7P7, Q9BQ44,
UniProt Related Accession:	P20338
Molecular Weight:	213
NCBI Full Name:	Ras-related protein Rab-4A
NCBI Synonym Full Names:	RAB4A, member RAS oncogene family
NCBI Official Symbol:	RAB4A
NCBI Official Synonym Symbols:	RAB4; HRES1; HRES-1; HRES-1/RAB4
NCBI Protein Information:	ras-related protein Rab-4A; HTLV-1 related endogenous sequence
UniProt Protein Name:	Ras-related protein Rab-4A
Protein Family:	Ras-related protein
UniProt Gene Name:	RAB4A
UniProt Entry Name:	RAB4A_HUMAN

Western blot analysis of extracts of various cell lines, using RAB4A antibody (CAB6712) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.

Antibodies

Anti-RAB4A Antibody (CAB13540)

Anti-RAB4A Antibody (CAB3979)

RAB4A Antibody (PACO11665)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)