Cell Biology Antibodies 11
Anti-RAB3IP Antibody (CAB8094)
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- SKU:
- CAB8094
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | RAB3IP Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8094 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human RAB3IP (NP_071901.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, A-549, 293T, Mouse heart, Mouse kidney, Mouse brain, Mouse liver, Rat brain, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human RAB3IP (NP_071901.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAND PLEG FHEV NLAS PTSP DLLG VYES GTQE QTTS PSVI YRPH PSAL SSVP IQAN ALDV SELP TQPV YSSP RRLN CAEI SSIS FHVT DPAP CSTS GVTA GLTK LTTR KDNY NAER EFLQ GATI TEAC DGSD DIFG LSTD SLSR LRSP SV |
| Gene ID: | 117177 |
| Uniprot: | Q96QF0 |
| Cellular Location: | Cell projection, Cytoplasm, Nucleus, cytoskeleton, lamellipodium |
| Calculated MW: | 28kDa/33-53kDa |
| Observed MW: | 53kDa |
| Synonyms: | RAB3IP, RABIN3, RABIN8 |
| Background: |
| UniProt Protein Function: | RAB3IP: Guanine nucleotide exchange factor for RAB8A. Mediates the release of GDP from RAB8A but not from RAB3A or RAB5. Modulates actin organization and promotes polarized transport of RAB8A-specific vesicles to the cell surface. Together with RAB11A, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Belongs to the SEC2 family. 8 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:GEF; GEF, Rab Chromosomal Location of Human Ortholog: 12q15 Cellular Component: cytosol; nucleus Molecular Function:protein binding; Rab guanyl-nucleotide exchange factor activity Biological Process: protein targeting to membrane |
| UniProt Code: | Q96QF0 |
| NCBI GenInfo Identifier: | 71152025 |
| NCBI Gene ID: | 117177 |
| NCBI Accession: | Q96QF0.1 |
| UniProt Secondary Accession: | Q96QF0,Q6PCE4, Q96A24, Q96QE6, Q96QE7, Q96QE8, Q96QE9 Q96QF1, Q9H673, B7WPJ6, |
| UniProt Related Accession: | Q96QF0 |
| Molecular Weight: | 53kDa |
| NCBI Full Name: | Rab-3A-interacting protein |
| NCBI Synonym Full Names: | RAB3A interacting protein |
| NCBI Official Symbol: | RAB3IP |
| NCBI Official Synonym Symbols: | RABIN3; RABIN8 |
| NCBI Protein Information: | rab-3A-interacting protein |
| UniProt Protein Name: | Rab-3A-interacting protein |
| UniProt Synonym Protein Names: | Rabin-3; SSX2-interacting protein |
| Protein Family: | Rab-3A-interacting protein |
| UniProt Gene Name: | RAB3IP |
 | Western blot analysis of extracts of various cell lines, using RAB3IP antibody (CAB8094) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded mouse brain using RAB3IP antibody (CAB8094) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat brain using RAB3IP antibody (CAB8094) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human lung cancer using RAB3IP antibody (CAB8094) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using RAB3IP antibody (CAB8094) at dilution of 1:100 (40x lens). |
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