Name: Anti-RAB35 Antibody (CAB8030)
Brand: Cell Biology Antibodies 11
SKU: CAB8030
Availability: OutOfStock

Antibody Name:	RAB35 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8030
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-201 of human RAB35 (NP_006852.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, Mouse brain, Mouse lung, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-201 of human RAB35 (NP_006852.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MARD YDHL FKLL IIGD SGVG KSSL LLRF ADNT FSGS YITT IGVD FKIR TVEI NGEK VKLQ IWDT AGQE RFRT ITST YYRG THGV IVVY DVTS AESF VNVK RWLH EINQ NCDD VCRI LVGN KNDD PERK VVET EDAY KFAG QMGI QLFE TSAK ENVN VEEM FNCI TELV LRAK KDNL AKQQ QQQQ NDVV KLTK NSKR KKRC C
Gene ID:	11021
Uniprot:	Q15286
Cellular Location:	Cell membrane, Cytoplasmic side, Cytoplasmic vesicle, Endosome, Lipid-anchor, Melanosome, Membrane, clathrin-coated pit, clathrin-coated vesicle
Calculated MW:	17kDa/23kDa
Observed MW:	34kDa

Synonyms:	RAB35, H-ray, RAB1C, RAY
Background:

UniProt Protein Function:	RAB35: In the process of endocytosis, essential rate-limiting regulator of a fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. Interacts with DENND1A and DENND1B in a nucleotide- dependent manner. The low binding to DENND1C is nucleotide- independent. All 3 DENND1 act as GEFs for RAB35 and thus activate the protein. Belongs to the small GTPase superfamily. Rab family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:G protein; G protein, monomeric, Rab; G protein, monomeric Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: cell projection membrane; clathrin-coated endocytic vesicle; coated pit; endoplasmic reticulum membrane; endosome membrane; Golgi membrane; intercellular bridge; plasma membrane Molecular Function:GDP binding; GTP binding; GTPase activity; phosphatidylinositol-4,5-bisphosphate binding; protein binding Biological Process: antigen processing and presentation; cytokinesis; endocytic recycling; endosome transport; ER to Golgi vesicle-mediated transport; neurite development; plasma membrane to endosome transport; protein localization
UniProt Code:	Q15286
NCBI GenInfo Identifier:	3024525
NCBI Gene ID:	11021
NCBI Accession:	Q15286.1
UniProt Secondary Accession:	Q15286,B2R6E0, B4E390,
UniProt Related Accession:	Q15286
Molecular Weight:	17,058 Da
NCBI Full Name:	Ras-related protein Rab-35
NCBI Synonym Full Names:	RAB35, member RAS oncogene family
NCBI Official Symbol:	RAB35
NCBI Official Synonym Symbols:	RAY; H-ray; RAB1C
NCBI Protein Information:	ras-related protein Rab-35
UniProt Protein Name:	Ras-related protein Rab-35
UniProt Synonym Protein Names:	GTP-binding protein RAY; Ras-related protein Rab-1C
Protein Family:	Ras-related protein
UniProt Gene Name:	RAB35
UniProt Entry Name:	RAB35_HUMAN

Anti-RAB35 Polyclonal Antibody (CAB8030)

Western blot analysis of extracts of various cell lines, using RAB35 antibody (CAB8030) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

Anti-RAB35 Antibody (CAB17091)

RAB35 Antibody (PACO01384)

RAB35 Antibody (PACO11651)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)