Cell Biology Antibodies 2
Anti-RAB25 Antibody (CAB12275)
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- SKU:
- CAB12275
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-RAB25 Antibody |
| Antibody SKU: | CAB12275 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-213 of human RAB25 (NP_065120.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse brain, Rat brain, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-213 of human RAB25 (NP_065120.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGNG TEED YNFV FKVV LIGE SGVG KTNL LSRF TRNE FSHD SRTT IGVE FSTR TVML GTAA VKAQ IWDT AGLE RYRA ITSA YYRG AVGA LLVF DLTK HQTY AVVE RWLK ELYD HAEA TIVV MLVG NKSD LSQA REVP TEEA RMFA ENNG LLFL ETSA LDST NVEL AFET VLKE IFAK VSKQ RQNS IRTN AITL GSAQ AGQE PGPG EKRA CCIS L |
| Gene ID: | 57111 |
| Uniprot: | P57735 |
| Cellular Location: | Cell membrane, Cell projection, Cytoplasmic side, Cytoplasmic vesicle, Lipid-anchor, pseudopodium membrane |
| Calculated MW: | 23kDa |
| Observed MW: | 23kDa |
| Synonyms: | RAB25, CATX-8, RAB11C |
| Background: | The protein encoded by this gene is a member of the RAS superfamily of small GTPases. The encoded protein is involved in membrane trafficking and cell survival. This gene has been found to be a tumor suppressor and an oncogene, depending on the context. Two variants, one protein-coding and the other not, have been found for this gene. |
| UniProt Protein Function: | RAB25: Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia. Increases the rates of ovarian and breast cancers progression and aggressiveness by modulating cellular processes such as proliferation, survival and migration of epithelial tumor cells. May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis. Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4. Interacts (via the hypervariable C-terminal region) with ITGB1 (via the cytoplasmic region); the interaction is GTP- dependent. Interacts with ITGAV. Associates with the integrin alpha-V/beta-1 heterodimer. Expressed in ovarian epithelium (NOE) and breast tissue. Expressed in ovarian cancer; expression is increased relative to NOE cells. Expression in ovarian cancer is stage dependent, with stage III and stage IV showing higher levels than early stage cancers. Expressed in breast cancer; expression is increased relative to normal breast tissue. Belongs to the small GTPase superfamily. Rab family. |
| UniProt Protein Details: | Protein type:G protein, monomeric; G protein, monomeric, Rab Chromosomal Location of Human Ortholog: 1q22 Cellular Component: cytoplasmic vesicle; pseudopodium Molecular Function:myosin V binding; protein binding Biological Process: positive regulation of cell proliferation; pseudopodium organization and biogenesis |
| NCBI Summary: | The protein encoded by this gene is a member of the RAS superfamily of small GTPases. The encoded protein is involved in membrane trafficking and cell survival. This gene has been found to be a tumor suppressor and an oncogene, depending on the context. Two variants, one protein-coding and the other not, have been found for this gene. [provided by RefSeq, Nov 2015] |
| UniProt Code: | P57735 |
| NCBI GenInfo Identifier: | 46577696 |
| NCBI Gene ID: | 57111 |
| NCBI Accession: | P57735.2 |
| UniProt Secondary Accession: | P57735,Q5VYA2, Q8NG24, Q96GB1, Q9BT12, |
| UniProt Related Accession: | P57735 |
| Molecular Weight: | 23kDa |
| NCBI Full Name: | Ras-related protein Rab-25 |
| NCBI Synonym Full Names: | RAB25, member RAS oncogene family |
| NCBI Official Symbol: | RAB25 |
| NCBI Official Synonym Symbols: | CATX-8; RAB11C |
| NCBI Protein Information: | ras-related protein Rab-25 |
| UniProt Protein Name: | Ras-related protein Rab-25 |
| UniProt Synonym Protein Names: | CATX-8 |
| Protein Family: | Ras-related protein |
| UniProt Gene Name: | RAB25 |
 | Western blot analysis of extracts of various cell lines, using RAB25 antibody (CAB12275) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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