KO Validated Antibodies 2

Anti-RAB1B Antibody (CAB19935)[KO Validated]

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SKU:
CAB19935
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Autophagy

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-RAB1B Antibody [KO Validated]
Antibody SKU:CAB19935
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human RAB1B.
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant protein of human RAB1B.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:81876
Uniprot:Q9H0U4
Cellular Location:Cytoplasm, Cytoplasmic side, Lipid-anchor, Membrane, Preautophagosomal structure membrane
Calculated MW:22kDa
Observed MW:22KDa
Anti-RAB1B Antibody [KO Validated] (CAB19935)Western blot analysis of extracts from normal (control) and RAB1B knockout (KO) HeLa cells, using RAB1B antibody (CAB19935) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:RAB1B
Background:
UniProt Protein Function:RAB1B: Protein transport. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Belongs to the small GTPase superfamily. Rab family.
UniProt Protein Details:

Protein type:G protein, monomeric; G protein, monomeric, Rab; G protein

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; mitochondrion; pre-autophagosomal structure membrane; ER-Golgi intermediate compartment

Molecular Function:GTPase activity; protein binding; GDP binding; GTP binding

Biological Process: intracellular protein transport; ER to Golgi vesicle-mediated transport; autophagy; virus assembly; Rab protein signal transduction

NCBI Summary:Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential for vesicle transport between the endoplasmic reticulum (ER) and Golgi (Chen et al., 1997 [PubMed 9030196]; Alvarez et al., 2003 [PubMed 12802079]).[supplied by OMIM, Jan 2009]
UniProt Code:Q9H0U4
NCBI GenInfo Identifier:23396834
NCBI Gene ID:81876
NCBI Accession:Q9H0U4.1
UniProt Secondary Accession:Q9H0U4,A8K7S1,
UniProt Related Accession:Q9H0U4
Molecular Weight:201
NCBI Full Name:Ras-related protein Rab-1B
NCBI Synonym Full Names:RAB1B, member RAS oncogene family
NCBI Official Symbol:RAB1B  
NCBI Protein Information:ras-related protein Rab-1B; small GTP-binding protein
UniProt Protein Name:Ras-related protein Rab-1B
Protein Family:Ras-related protein
UniProt Gene Name:RAB1B  
UniProt Entry Name:RAB1B_HUMAN
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