Anti-RAB11A/B Antibody (CAB20996)
- SKU:
- CAB20996
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-RAB11A/B Antibody (CAB20996) |
| Antibody SKU: | CAB20996 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunohistochemistry |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RAB11A/B (NP_004654.1). |
| Application: | Western blotting, Immunohistochemistry |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, C6, Neuro-2a |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RAB11A/B (NP_004654.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGTR DDEY DYLF KVVL IGDS GVGK SNLL SRFT RNEF NLES KSTI GVEF ATRS IQVD GKTI KAQI WDTA GQER YRAI TSAY YRGA VGAL LVYD IAKH LTYE |
| Cellular Location: | |
| Calculated MW: | |
| Observed MW: | 24KDa |
| Synonyms: | |
| Background: |
 | Western blot analysis of extracts of various cell lines, using RAB11A/B antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using RAB11A/B Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Western blot analysis of extracts of A-549 cells, using RAB11A/B antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
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