Anti-RAB11A/B Antibody (CAB20996)

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SKU:
CAB20996

Description

Antibody Name:Anti-RAB11A/B Antibody (CAB20996)
Antibody SKU:CAB20996
Antibody Size:50µL, 100µL
Application:Western blotting, Immunohistochemistry
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RAB11A/B (NP_004654.1).
Application:Western blotting, Immunohistochemistry
Recommended Dilution:WB 1:500 - 1:2000
IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, C6, Neuro-2a
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RAB11A/B (NP_004654.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGTR DDEY DYLF KVVL IGDS GVGK SNLL SRFT RNEF NLES KSTI GVEF ATRS IQVD GKTI KAQI WDTA GQER YRAI TSAY YRGA VGAL LVYD IAKH LTYE
Cellular Location:
Calculated MW:
Observed MW:24KDa
Synonyms:
Background:
Western blot analysis of extracts of various cell lines, using RAB11A/B antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Western blot analysis of extracts of various cell lines, using RAB11A/B antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry of paraffin-embedded human liver cancer using RAB11A/B Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded human liver cancer using RAB11A/B Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Western blot analysis of extracts of A-549 cells, using RAB11A/B antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Western blot analysis of extracts of A-549 cells, using RAB11A/B antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
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Additional Information

Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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