KO Validated Antibodies 1

Anti-RAB11A Antibody (CAB18048)[KO Validated]

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SKU:
CAB18048
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-RAB11A Antibody [KO Validated]
Antibody SKU:CAB18048
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human RAB11A.
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant protein of human RAB11A.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:8766
Uniprot:P62491
Cellular Location:
Calculated MW:24kDa
Observed MW:25kDa
Anti-RAB11A Antibody [KO Validated] (CAB18048)Western blot analysis of extracts from normal (control) and RAB11A knockout (KO) HeLa cells, using RAB11A antibody (CAB18048) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Synonyms:RAB11A, YL8
Background:The protein encoded by this gene belongs to the Rab family of the small GTPase superfamily. It is associated with both constitutive and regulated secretory pathways, and may be involved in protein transport. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:RAB11A: Regulates endocytic recycling. May exert its functions by interacting with multiple effector proteins in different complexes. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. Interacts with RIP11 and STXBP6. Interacts with SGSM1, SGSM2 and SGSM3. Interacts with EXOC6 in a GTP- dependent manner. Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 (via its C-terminus) and RAB11FIP4. Interacts with EVI5; EVI5 and RAB11FIP3 may be mutually exclusive and compete for binding RAB11A. Interacts with SPE39//C14orf133. Interacts with MYO5B. Found in a complex with MYO5B and CFTR. Interacts with NPC1L1. Interacts (GDP-bound form) with ZFYVE27. Interacts with BIRC6/bruce. Belongs to the small GTPase superfamily. Rab family.
UniProt Protein Details:

Protein type:G protein, monomeric; G protein; G protein, monomeric, Rab; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q22.31

Cellular Component: Golgi apparatus; spindle pole; protein complex; cytoplasmic vesicle membrane; mitochondrion; recycling endosome membrane; phagocytic vesicle; trans-Golgi network; cytosol; kinetochore microtubule; multivesicular body; recycling endosome; phagocytic vesicle membrane; transport vesicle; perinuclear region of cytoplasm; axon; plasma membrane; cytoplasmic vesicle; vesicle; cleavage furrow

Molecular Function:GTPase activity; protein binding; GDP binding; syntaxin binding; GTP binding; microtubule binding; myosin V binding

Biological Process: regulation of long-term neuronal synaptic plasticity; establishment of vesicle localization; exocytosis; metabolic process; organelle organization and biogenesis; positive regulation of axon extension; water transport; cytokinesis; melanosome transport; mitotic metaphase plate congression; vesicle-mediated transport; regulation of protein transport; intracellular protein transport; astral microtubule organization and biogenesis; renal water homeostasis; plasma membrane to endosome transport; Rab protein signal transduction; transmembrane transport; neurite development

NCBI Summary:The protein encoded by this gene belongs to the Rab family of the small GTPase superfamily. It is associated with both constitutive and regulated secretory pathways, and may be involved in protein transport. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]
UniProt Code:P62491
NCBI GenInfo Identifier:50402542
NCBI Gene ID:8766
NCBI Accession:P62491.3
UniProt Secondary Accession:P62491,P24410, Q5TZN9, Q9JLX1, B2R4B6, B4DT13,
UniProt Related Accession:P62491
Molecular Weight:17,659 Da
NCBI Full Name:Ras-related protein Rab-11A
NCBI Synonym Full Names:RAB11A, member RAS oncogene family
NCBI Official Symbol:RAB11A  
NCBI Official Synonym Symbols:YL8  
NCBI Protein Information:ras-related protein Rab-11A; rab-11; RAB 11A, member oncogene family
UniProt Protein Name:Ras-related protein Rab-11A
UniProt Synonym Protein Names:YL8
Protein Family:Ras-related protein
UniProt Gene Name:RAB11A  
UniProt Entry Name:RB11A_HUMAN
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