Metabolism Antibodies 3

Anti-QRSL1 Antibody (CAB8499)

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SKU:
CAB8499
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

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Antibody Name:Anti-QRSL1 Antibody
Antibody SKU:CAB8499
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 289-528 of human QRSL1 (NP_060762.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HeLa, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 289-528 of human QRSL1 (NP_060762.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ELSS EVQS LWSK AADL FESE GAKV IEVS LPHT SYSI VCYH VLCT SEVA SNMA RFDG LQYG HRCD IDVS TEAM YAAT RREG FNDV VRGR ILSG NFFL LKEN YENY FVKA QKVR RLIA NDFV NAFN SGVD VLLT PTTL SEAV PYLE FIKE DNRT RSAQ DDIF TQAV NMAG LPAV SIPV ALSN QGLP IGLQ FIGR AFCD QQLL TVAK WFEK QVQF PVIQ LQEL MDDC SAVL ENEK LASV SLKQ
Gene ID:55278
Uniprot:Q9H0R6
Cellular Location:Mitochondrion
Calculated MW:32kDa/57kDa
Observed MW:57kDa
Synonyms:QRSL1, GatA
Background:
UniProt Protein Function:QRSL1: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu- tRNA(Gln). Belongs to the amidase family. GatA subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 6.3.5.7; Ligase

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: mitochondrion

Molecular Function:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity

Biological Process: mitochondrial translation

UniProt Code:Q9H0R6
NCBI GenInfo Identifier:167016573
NCBI Gene ID:55278
NCBI Accession:Q9H0R6.2
UniProt Secondary Accession:Q9H0R6,Q5VWJ4, Q9HA60, Q9NV19,
UniProt Related Accession:Q9H0R6
Molecular Weight:57kDa
NCBI Full Name:Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial
NCBI Synonym Full Names:glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1
NCBI Official Symbol:QRSL1  
NCBI Official Synonym Symbols:GatA  
NCBI Protein Information:glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial
UniProt Protein Name:Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial
UniProt Synonym Protein Names:Glutaminyl-tRNA synthase-like protein 1
UniProt Gene Name:QRSL1  
Anti-QRSL1 Polyclonal Antibody (CAB8499)Western blot analysis of extracts of various cell lines, using QRSL1 antibody (CAB8499) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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