Developmental Biology
Anti-QKI Antibody (CAB7043)
- SKU:
- CAB7043
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-QKI Antibody |
| Antibody SKU: | CAB7043 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-341 of human QKI (NP_006766.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HL-60, HeLa, NCI-H460, Mouse heart, Mouse lung, Mouse brain, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-341 of human QKI (NP_006766.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MVGE METK EKPK PTPD YLMQ LMND KKLM SSLP NFCG IFNH LERL LDEE ISRV RKDM YNDT LNGS TEKR SAEL PDAV GPIV QLQE KLYV PVKE YPDF NFVG RILG PRGL TAKQ LEAE TGCK IMVR GKGS MRDK KKEE QNRG KPNW EHLN EDLH VLIT VEDA QNRA EIKL KRAV EEVK KLLV PAAE GEDS LKKM QLME LAIL NGTY RDAN IKSP ALAF SLAA TAQA APRI ITGP APVL PPAA LRTP TPAG PTIM PLIR QIQT AVMP NGTP HPTA AIVP PGPE AGLI YTPY EYPY TLAP ATSI LEYP IEPS GVLG AVAT KVRR HDMR VHPY QRIV TADR AATG N |
| Gene ID: | 9444 |
| Uniprot: | Q96PU8 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 35kDa/36kDa/37kDa |
| Observed MW: | 38kDa |
| Synonyms: | QKI, Hqk, QK, QK1, QK3, hqkI |
| Background: | The protein encoded by this gene is an RNA-binding protein that regulates pre-mRNA splicing, export of mRNAs from the nucleus, protein translation, and mRNA stability. The encoded protein is involved in myelinization and oligodendrocyte differentiation and may play a role in schizophrenia. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | RNA-binding protein that plays a central role in myelinization (PubMed:16641098). Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Regulates target mRNA stability (PubMed:23630077). In addition, acts by regulating pre-mRNA splicing, mRNA export and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B. Regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia (PubMed:16641098). Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor. |
| NCBI Summary: | The protein encoded by this gene is an RNA-binding protein that regulates pre-mRNA splicing, export of mRNAs from the nucleus, protein translation, and mRNA stability. The encoded protein is involved in myelinization and oligodendrocyte differentiation and may play a role in schizophrenia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014] |
| UniProt Code: | Q96PU8 |
| NCBI GenInfo Identifier: | 74761039 |
| NCBI Gene ID: | 9444 |
| NCBI Accession: | Q96PU8.1 |
| UniProt Secondary Accession: | Q96PU8,Q2I375, Q5MJQ1, Q969L9, Q96EJ3, Q96KA3, Q96PU6 Q96PU7, Q9P0X6, Q9P0X7, Q9P0X8, Q9P0X9, |
| UniProt Related Accession: | Q96PU8 |
| Molecular Weight: | 35,131 Da |
| NCBI Full Name: | Protein quaking |
| NCBI Synonym Full Names: | QKI, KH domain containing RNA binding |
| NCBI Official Symbol: | QKI |
| NCBI Official Synonym Symbols: | QK; Hqk; QK1; QK3; hqkI |
| NCBI Protein Information: | protein quaking |
| UniProt Protein Name: | Protein quaking |
| Protein Family: | Protein quaking |
| UniProt Gene Name: | QKI |
| UniProt Entry Name: | QKI_HUMAN |
 | Western blot analysis of extracts of various cell lines, using QKI antibody (CAB7043) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s. |
 | Western blot analysis of extracts of various cell lines, using QKI antibody (CAB7043) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse brain using QKI antibody (CAB7043) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of MCF7 cells using QKI antibody (CAB7043). |
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