Neuroscience

Anti-PZP Antibody (CAB3324)

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SKU:
CAB3324
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Neuroscience

Description

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Antibody Name:Anti-PZP Antibody
Antibody SKU:CAB3324
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 620-740 of human PZP (NP_002855.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, Mouse liver, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 620-740 of human PZP (NP_002855.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VKDL TNFP DNVD QQEE EQGH CPRP FFIH NGAI YVPL SSNE ADIY SFLK GMGL KVFT NSKI RKPK SCSV IPSV SAGA VGQG YYGA GLGV VERP YVPQ LGTY NVIP LNNE QSSG PVPE TVRS Y
Gene ID:5858
Uniprot:P20742
Cellular Location:Secreted
Calculated MW:140kDa/163kDa
Observed MW:164kDa
Synonyms:PZP, CPAMD6
Background:The protein encoded by this gene is highly expressed in late-pregnancy serum and is similar in structure to alpha-2-macroglobulin. The encoded protein, which acts as a homotetramer, inhibits the activity of all four classes of proteinases. This protein contains cleavage sites for several proteinases. Upon binding of a proteinase, the conformation of this protein changes to trap the proteinase, limiting its activity. This protein appears to be elevated in the sera of presymptomatic Alzheimer's disease patients.
UniProt Protein Function:PZP: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12p13-p12.2

Cellular Component: extracellular region

Molecular Function:endopeptidase inhibitor activity; serine-type endopeptidase inhibitor activity

Biological Process: female pregnancy

NCBI Summary:The protein encoded by this gene is highly expressed in late-pregnancy serum and is similar in structure to alpha-2-macroglobulin. The encoded protein, which acts as a homotetramer, inhibits the activity of all four classes of proteinases. This protein contains cleavage sites for several proteinases. Upon binding of a proteinase, the conformation of this protein changes to trap the proteinase, limiting its activity. This protein appears to be elevated in the sera of presymptomatic Alzheimer's disease patients. [provided by RefSeq, Dec 2016]
UniProt Code:P20742
NCBI GenInfo Identifier:281185515
NCBI Gene ID:
NCBI Accession:P20742.4
UniProt Secondary Accession:P20742,Q15273, Q2NKL2, Q7M4N7, A6ND27,
Molecular Weight:42.3 kDa
NCBI Full Name:Pregnancy zone protein
UniProt Protein Name:Pregnancy zone protein
UniProt Synonym Protein Names:C3 and PZP-like alpha-2-macroglobulin domain-containing protein 6
Protein Family:Pregnancy zone protein
UniProt Gene Name:PZP  
UniProt Entry Name:PZP_HUMAN
Anti-PZP Antibody (CAB3324)Western blot analysis of extracts of various cell lines, using PZP antibody (CAB3324) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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