Metabolism Antibodies 2

Anti-PYGB Antibody (CAB6402)

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SKU:
CAB6402
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-PYGB Antibody
Antibody SKU:CAB6402
Antibody Size:20uL, 50uL, 100uL
Application:WB IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 544-843 of human PYGB (NP_002853.2).
Application:WB IF IP
Recommended Dilution:WB 1:500 - 1:2000 IF 1:20 - 1:100 IP 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:BT-474, SW620, HT-29, HepG2, Mouse brain, Mouse heart, Mouse lung, Rat brain, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 544-843 of human PYGB (NP_002853.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LKFS AFLE KEYK VKIN PSSM FDVH VKRI HEYK RQLL NCLH VVTL YNRI KRDP AKAF VPRT VMIG GKAA PGYH MAKL IIKL VTSI GDVV NHDP VVGD RLKV IFLE NYRV SLAE KVIP AADL SQQI STAG TEAS GTGN MKFM LNGA LTIG TMDG ANVE MAEE AGAE NLFI FGLR VEDV EALD RKGY NARE YYDH LPEL KQAV DQIS SGFF SPKE PDCF KDIV NMLM HHDR FKVF ADYE AYMQ CQAQ VDQL YRNP KEWT KKVI RNIA CSGK FSSD RTIT EYAR EIWG VEPS DLQI PPPN IPRD
Gene ID:5834
Uniprot:P11216
Cellular Location:
Calculated MW:96kDa
Observed MW:105kDa
Synonyms:PYGB, GPBB
Background:The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation.
UniProt Protein Function:PYGB: Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Belongs to the glycogen phosphorylase family.
UniProt Protein Details:

Protein type:Phosphorylase; Kinase, other; Carbohydrate Metabolism - starch and sucrose; Transferase; EC 2.4.1.1

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: cytoplasm; membrane

Molecular Function:glycogen phosphorylase activity; protein binding; pyridoxal phosphate binding

Biological Process: glycogen catabolic process

NCBI Summary:The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. [provided by RefSeq, Jul 2008]
UniProt Code:P11216
NCBI GenInfo Identifier:20178317
NCBI Gene ID:5834
NCBI Accession:P11216.5
UniProt Secondary Accession:P11216,Q96AK1, Q9NPX8,
UniProt Related Accession:P11216
Molecular Weight:96,696 Da
NCBI Full Name:Glycogen phosphorylase, brain form
NCBI Synonym Full Names:phosphorylase, glycogen; brain
NCBI Official Symbol:PYGB  
NCBI Official Synonym Symbols:GPBB  
NCBI Protein Information:glycogen phosphorylase, brain form
UniProt Protein Name:Glycogen phosphorylase, brain form
Protein Family:Glycogen phosphorylase
UniProt Gene Name:PYGB  
UniProt Entry Name:PYGB_HUMAN
Anti-PYGB Antibody (CAB6402)Western blot analysis of extracts of various cell lines, using PYGB antibody (CAB6402) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-PYGB Antibody (CAB6402)Immunofluorescence analysis of MCF-7 cells using PYGB antibody (CAB6402). Blue: DAPI for nuclear staining.
Anti-PYGB Antibody (CAB6402)Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug PYGB antibody (CAB6402). Western blot was performed from the immunoprecipitate using PYGB antibody (CAB6402) at a dilition of 1:1000.
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