Metabolism Antibodies 1
Anti-PYCR2 Antibody (CAB15155)
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- SKU:
- CAB15155
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-PYCR2 Antibody |
| Antibody SKU: | CAB15155 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 271-320 of human PYCR2 (NP_037460.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, A-431, HT-29, Mouse brain, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 271-320 of human PYCR2 (NP_037460.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MADQ EKIS PAAL KKTL LDRV KLES PTVS TLTP SSPG KLLT RSLA LGGK KD |
| Gene ID: | 29920 |
| Uniprot: | Q96C36 |
| Cellular Location: | Cytoplasm, Mitochondrion |
| Calculated MW: | 33kDa |
| Observed MW: | 35kDa |
| Synonyms: | PYCR2, HLD10, P5CR2 |
| Background: | This gene belongs to the pyrroline-5-carboxylate reductase family. The encoded mitochondrial protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. Alternatively spliced transcript variants have been described for this gene. |
| UniProt Protein Function: | PYCR2: Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+). Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH. Belongs to the pyrroline-5-carboxylate reductase family. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - arginine and proline; Oxidoreductase; EC 1.5.1.2 Chromosomal Location of Human Ortholog: 1q42.12 Cellular Component: cytoplasm Molecular Function:pyrroline-5-carboxylate reductase activity Disease: Leukodystrophy, Hypomyelinating, 10 |
| NCBI Summary: | This gene belongs to the pyrroline-5-carboxylate reductase family. The encoded mitochondrial protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Nov 2012] |
| UniProt Code: | Q96C36 |
| NCBI GenInfo Identifier: | 60390642 |
| NCBI Gene ID: | 29920 |
| NCBI Accession: | Q96C36.1 |
| UniProt Related Accession: | Q96C36 |
| Molecular Weight: | |
| NCBI Full Name: | Pyrroline-5-carboxylate reductase 2 |
| NCBI Synonym Full Names: | pyrroline-5-carboxylate reductase 2 |
| NCBI Official Symbol: | PYCR2 |
| NCBI Official Synonym Symbols: | HLD10; P5CR2 |
| NCBI Protein Information: | pyrroline-5-carboxylate reductase 2 |
| UniProt Protein Name: | Pyrroline-5-carboxylate reductase 2 |
| Protein Family: | Pyrroline-5-carboxylate reductase |
| UniProt Gene Name: | PYCR2 |
| UniProt Entry Name: | P5CR2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PYCR2 antibody (CAB15155) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded Mouse kidney using PYCR2 Rabbit pAb (CAB15155) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Rat liver using PYCR2 Rabbit pAb (CAB15155) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Human liver cancer using PYCR2 Rabbit pAb (CAB15155) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using PYCR2 antibody (CAB15155) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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