Metabolism Antibodies 2

Anti-PYCR1 Antibody (CAB6959)

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SKU:
CAB6959
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-PYCR1 Antibody
Antibody SKU:CAB6959
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-319 of human PYCR1 (NP_008838.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:BT-474, HepG2, Mouse skeletal muscle, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-319 of human PYCR1 (NP_008838.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSVG FIGA GQLA FALA KGFT AAGV LAAH KIMA SSPD MDLA TVSA LRKM GVKL TPHN KETV QHSD VLFL AVKP HIIP FILD EIGA DIED RHIV VSCA AGVT ISSI EKKL SAFR PAPR VIRC MTNT PVVV REGA TVYA TGTH AQVE DGRL MEQL LSSV GFCT EVEE DLID AVTG LSGS GPAY AFTA LDAL ADGG VKMG LPRR LAVR LGAQ ALLG AAKM LLHS EQHP GQLK DNVS SPGG ATIH ALHV LESG GFRS LLIN AVEA SCIR TREL QSMA DQEQ VSPA AIKK TILD KVKL DSPA GTAL SPSG HTKL LPRS LAPA GKD
Gene ID:5831
Uniprot:P32322
Cellular Location:Mitochondrion
Calculated MW:33kDa/35kDa
Observed MW:36kDa
Synonyms:PYCR1, ARCL2B, ARCL3B, P5C, P5CR, PIG45, PP222, PRO3, PYCR
Background:This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:PYCR1: Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress. Defects in PYCR1 are the cause of cutis laxa autosomal recessive type 2B (ARCL2B). A multisystem disorder characterized by the appearance of premature aging, wrinkled and lax skin with reduced elasticity, joint laxity, craniofacial dysmorphic features, intrauterine growth retardation with some degree of postnatal growth deficiency, and developmental delay. Defects in PYCR1 are the cause of cutis laxa, autosomal recessive, type 3B (ARCL3B). ARCL3B is a disorder characterized by an aged appearance with distinctive facial features, sparse hair, ophthalmologic abnormalities, intrauterine growth retardation, and cutis laxa. Belongs to the pyrroline-5-carboxylate reductase family.
UniProt Protein Details:

Protein type:EC 1.5.1.2; Oxidoreductase; Mitochondrial; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function:identical protein binding; protein binding; pyrroline-5-carboxylate reductase activity

Biological Process: amino acid biosynthetic process; proline biosynthetic process; regulation of mitochondrial membrane potential

Disease: Cutis Laxa, Autosomal Recessive, Type Iib; Cutis Laxa, Autosomal Recessive, Type Iiib

NCBI Summary:This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
UniProt Code:P32322
NCBI GenInfo Identifier:60416434
NCBI Gene ID:5831
NCBI Accession:P32322.2
UniProt Secondary Accession:P32322,Q6FHI4, Q96DI6, Q9HBQ4, A6NFM2, B4DMU0,
UniProt Related Accession:P32322
Molecular Weight:35,981 Da
NCBI Full Name:Pyrroline-5-carboxylate reductase 1, mitochondrial
NCBI Synonym Full Names:pyrroline-5-carboxylate reductase 1
NCBI Official Symbol:PYCR1  
NCBI Official Synonym Symbols:P5C; P5CR; PRO3; PYCR; PIG45; PP222; ARCL2B; ARCL3B  
NCBI Protein Information:pyrroline-5-carboxylate reductase 1, mitochondrial
UniProt Protein Name:Pyrroline-5-carboxylate reductase 1, mitochondrial
Protein Family:Pyrroline-5-carboxylate reductase
UniProt Gene Name:PYCR1  
UniProt Entry Name:P5CR1_HUMAN
Anti-PYCR1 Antibody (CAB6959)Western blot analysis of extracts of various cell lines, using PYCR1 antibody (CAB6959) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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