Epigenetics & Nuclear Signaling Antibodies 2

Anti-PUS10 Antibody (CAB15962)

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SKU:
CAB15962
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-PUS10 Antibody
Antibody SKU:CAB15962
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 290-529 of human PUS10 (NP_653310.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse thymus, Mouse spleen, Mouse testis, Rat thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 290-529 of human PUS10 (NP_653310.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VAGR YNKY SRNL PQTP WIID GERK LESS VEEL ISDH LLAV FKAE SFNF SSSG REDV DVRT LGNG RPFA IELV NPHR VHFT SQEI KELQ QKIN NSSN KIQV RDLQ LVTR EAIG HMKE GEEE KTKT YSAL IWTN KAIQ KKDI EFLN DIKD LKID QKTP LRVL HRRP LAVR ARVI HFME TQYV DEHH FRLH LKTQ AGTY IKEF VHGD FGRT KPNI GSLM NVTA DILE LDVE SVDV DWPP ALDD
Gene ID:150962
Uniprot:Q3MIT2
Cellular Location:
Calculated MW:60kDa
Observed MW:60kDa
Synonyms:PUS10, CCDC139, DOBI
Background:Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).
UniProt Protein Function:PUS10: Pseudouridylate synthases catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. PUS10 catalyzes the formation of the universal psi55 in the GC loop of transfer RNAs (Probable). Modulator of TRAIL-induced cell death via activation of procaspase 8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death. Belongs to the pseudouridine synthase Pus10 family.
UniProt Protein Details:

Protein type:RNA processing; Isomerase; EC 5.4.99.-

Chromosomal Location of Human Ortholog: 2p16.1

Molecular Function:RNA binding; pseudouridine synthase activity

Biological Process: pseudouridine synthesis; tRNA processing

NCBI Summary:Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).[supplied by OMIM, May 2009]
UniProt Code:Q3MIT2
NCBI GenInfo Identifier:207113130
NCBI Gene ID:150962
NCBI Accession:NP_653310.2
UniProt Secondary Accession:Q3MIT2,Q5JPJ5, Q96MI8,
UniProt Related Accession:Q3MIT2
Molecular Weight:60,244 Da
NCBI Full Name:putative tRNA pseudouridine synthase Pus10
NCBI Synonym Full Names:pseudouridylate synthase 10
NCBI Official Symbol:PUS10  
NCBI Official Synonym Symbols:DOBI; CCDC139  
NCBI Protein Information:putative tRNA pseudouridine synthase Pus10
UniProt Protein Name:Putative tRNA pseudouridine synthase Pus10
UniProt Synonym Protein Names:Coiled-coil domain-containing protein 139; tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase
Protein Family:Putative tRNA pseudouridine synthase
UniProt Gene Name:PUS10  
UniProt Entry Name:PUS10_HUMAN
Anti-PUS10 Antibody (CAB15962)Western blot analysis of extracts of various cell lines, using PUS10 antibody (CAB15962) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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