Epigenetics & Nuclear Signaling Antibodies 4

Anti-PURA Antibody (CAB9296)

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SKU:
CAB9296
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-PURA Antibody
Antibody SKU:CAB9296
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 203-322 of human PURA (NP_005850.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:MCF7, A-549, HeLa, HepG2, Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 203-322 of human PURA (NP_005850.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AKLI DDYG VEEE PAEL PEGT SLTV DNKR FFFD VGSN KYGV FMRV SEVK PTYR NSIT VPYK VWAK FGHT FCKY SEEM KKIQ EKQR EKRA ACEQ LHQQ QQQQ QEET AAAT LLLQ GEEE GEED
Gene ID:5813
Uniprot:Q00577
Cellular Location:Nucleus
Calculated MW:34kDa
Observed MW:36kDa
Synonyms:PURA, MRD31, PUR-ALPHA, PUR1, PURALPHA
Background:This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia.
UniProt Protein Function:PURA: This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. May play a role in the initiation of DNA replication and in recombination. Belongs to the PUR DNA-binding protein family.
UniProt Protein Details:

Protein type:DNA-binding; RNA-binding

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: DNA replication factor A complex; nuclear chromosome, telomeric region; nucleus

Molecular Function:double-stranded telomeric DNA binding; protein binding; RNA polymerase II transcription factor activity, enhancer binding; single-stranded DNA binding; transcription factor binding

Biological Process: DNA replication initiation; DNA unwinding during replication

Disease: Mental Retardation, Autosomal Dominant 31

NCBI Summary:This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq, Jul 2008]
UniProt Code:Q00577
NCBI GenInfo Identifier:1346918
NCBI Gene ID:5813
NCBI Accession:Q00577.2
UniProt Related Accession:Q00577
Molecular Weight:34,911 Da
NCBI Full Name:Transcriptional activator protein Pur-alpha
NCBI Synonym Full Names:purine rich element binding protein A
NCBI Official Symbol:PURA  
NCBI Official Synonym Symbols:PUR1; MRD31; PURALPHA; PUR-ALPHA  
NCBI Protein Information:transcriptional activator protein Pur-alpha
UniProt Protein Name:Transcriptional activator protein Pur-alpha
UniProt Synonym Protein Names:Purine-rich single-stranded DNA-binding protein alpha
Protein Family:Puratrophin
UniProt Gene Name:PURA  
UniProt Entry Name:PURA_HUMAN
Anti-PURA Polyclonal Antibody (CAB9296)Western blot analysis of extracts of various cell lines, using PURA antibody (CAB9296) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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