Metabolism Antibodies 1

Anti-PTS Antibody (CAB14055)

(No reviews yet) Write a Review
SKU:
CAB14055
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-PTS Antibody
Antibody SKU:CAB14055
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human PTS (NP_000308.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, U-251MG, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human PTS (NP_000308.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSTE GGGR RCQA QVSR RISF SASH RLYS KFLS DEEN LKLF GKCN NPNG HGHN YKVV VTVH GEID PATG MVMN LADL KKYM EEAI MQPL DHKN LDMD VPYF ADVV STTE NVAV YIWD NLQK VLPV GVLY KVKV YETD NNIV VYKG E
Gene ID:5805
Uniprot:Q03393
Cellular Location:
Calculated MW:16kDa
Observed MW:16kDa
Synonyms:PTS, PTPS
Background:The enzyme encoded by this gene catalyzes the elimination of inorganic triphosphate from dihydroneopterin triphosphate, which is the second and irreversible step in the biosynthesis of tetrahydrobiopterin from GTP. Tetrahydrobiopterin, also known as BH(4), is an essential cofactor and regulator of various enzyme activities, including enzymes involved in serotonin biosynthesis and NO synthase activity. Mutations in this gene result in hyperphenylalaninemia.
UniProt Protein Function:PTS: Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6- pyruvoyl tetrahydropterin. Defects in PTS are the cause of BH4-deficient hyperphenylalaninemia type A (HPABH4A); also called 6-pyruvoyl-tetrahydropterin synthase deficiency (PTS deficiency) or hyperphenylalaninemia tetrahydrobiopterin-deficient due to PTS deficiency. HPABH4A is an autosomal recessive disorder characterized by depletion of the neurotransmitters dopamine and serotonin, and clinically by severe neurological symptoms unresponsive to the classic phenylalanine-low diet. Belongs to the PTPS family.
UniProt Protein Details:

Protein type:Mitochondrial; Cofactor and Vitamin Metabolism - folate biosynthesis; Lyase; EC 4.2.3.12

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: mitochondrion; cytoplasm; cytosol

Molecular Function:identical protein binding; protein homodimerization activity; metal ion binding; 6-pyruvoyltetrahydropterin synthase activity

Biological Process: amino acid metabolic process; tetrahydrobiopterin biosynthetic process; central nervous system development; regulation of nitric-oxide synthase activity; nitric oxide metabolic process

Disease: Hyperphenylalaninemia, Bh4-deficient, A

NCBI Summary:The enzyme encoded by this gene catalyzes the elimination of inorganic triphosphate from dihydroneopterin triphosphate, which is the second and irreversible step in the biosynthesis of tetrahydrobiopterin from GTP. Tetrahydrobiopterin, also known as BH(4), is an essential cofactor and regulator of various enzyme activities, including enzymes involved in serotonin biosynthesis and NO synthase activity. Mutations in this gene result in hyperphenylalaninemia. [provided by RefSeq, Oct 2008]
UniProt Code:Q03393
NCBI GenInfo Identifier:417553
NCBI Gene ID:5805
NCBI Accession:Q03393.1
UniProt Secondary Accession:Q03393,Q8WVG8, B0YJ87,
UniProt Related Accession:Q03393
Molecular Weight:145
NCBI Full Name:6-pyruvoyl tetrahydrobiopterin synthase
NCBI Synonym Full Names:6-pyruvoyltetrahydropterin synthase
NCBI Official Symbol:PTS  
NCBI Official Synonym Symbols:PTPS  
NCBI Protein Information:6-pyruvoyl tetrahydrobiopterin synthase; PTP synthase
UniProt Protein Name:6-pyruvoyl tetrahydrobiopterin synthase
Protein Family:PTS system EIIBC component
UniProt Gene Name:PTS  
UniProt Entry Name:PTPS_HUMAN
Anti-PTS Antibody (CAB14055)Western blot analysis of extracts of various cell lines, using PTS antibody (CAB14055) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-PTS Antibody (CAB14055)Immunofluorescence analysis of MCF-7 cells using PTS antibody (CAB14055). Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products