Antibody Name:	Anti-PTRH2 Antibody
Antibody SKU:	CAB6466
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 40-179 of human PTRH2 (NP_057161.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, BT-474, SW480, Mouse liver, Mouse spleen, Mouse thymus, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 40-179 of human PTRH2 (NP_057161.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LPKS KTSK THTD TESE ASIL GDSG EYKM ILVV RNDL KMGK GKVA AQCS HAAV SAYK QIQR RNPE MLKQ WEYC GQPK VVVK APDE ETLI ALLA HAKM LGLT VSLI QDAG RTQI APGS QTVL GIGP GPAD LIDK VTGH LKLY
Gene ID:	51651
Uniprot:	Q9Y3E5
Cellular Location:	Mitochondrion
Calculated MW:	19kDa
Observed MW:	19kDa

Synonyms:

PTRH2, BIT1, CFAP37, CGI-147, IMNEPD, PTH, PTH 2, PTH2

Background:

The protein encoded by this gene is a mitochondrial protein with two putative domains, an N-terminal mitochondrial localization sequence, and a UPF0099 domain. In vitro assays suggest that this protein possesses peptidyl-tRNA hydrolase activity, to release the peptidyl moiety from tRNA, thereby preventing the accumulation of dissociated peptidyl-tRNA that could reduce the efficiency of translation. This protein also plays a role regulating cell survival and death. It promotes survival as part of an integrin-signaling pathway for cells attached to the extracellular matrix (ECM), but also promotes apoptosis in cells that have lost their attachment to the ECM, a process called anoikos. After loss of cell attachment to the ECM, this protein is phosphorylated, is released from the mitochondria into the cytosol, and promotes caspase-independent apoptosis through interactions with transcriptional regulators. This gene has been implicated in the development and progression of tumors, and mutations in this gene have been associated with an infantile multisystem neurologic, endocrine, and pancreatic disease (INMEPD) characterized by intellectual disability, postnatal microcephaly, progressive cerebellar atrophy, hearing impairment, polyneuropathy, failure to thrive, and organ fibrosis with exocrine pancreas insufficiency (PMID: 25574476). Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	PTRH2: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis. Monomer. Belongs to the PTH2 family.
UniProt Protein Details:	Protein type:EC 3.1.1.29; Mitochondrial; Hydrolase Chromosomal Location of Human Ortholog: 17q23.1 Cellular Component: membrane; mitochondrion; cytosol Molecular Function:protein binding; aminoacyl-tRNA hydrolase activity Biological Process: apoptosis; metabolic process Disease: Neurologic, Endocrine, And Pancreatic Disease, Multisystem, Infantile-onset
NCBI Summary:	The protein encoded by this gene is a mitochondrial protein with two putative domains, an N-terminal mitochondrial localization sequence, and a UPF0099 domain. In vitro assays suggest that this protein possesses peptidyl-tRNA hydrolase activity, to release the peptidyl moiety from tRNA, thereby preventing the accumulation of dissociated peptidyl-tRNA that could reduce the efficiency of translation. This protein also plays a role regulating cell survival and death. It promotes survival as part of an integrin-signaling pathway for cells attached to the extracellular matrix (ECM), but also promotes apoptosis in cells that have lost their attachment to the ECM, a process called anoikos. After loss of cell attachment to the ECM, this protein is phosphorylated, is released from the mitochondria into the cytosol, and promotes caspase-independent apoptosis through interactions with transcriptional regulators. This gene has been implicated in the development and progression of tumors, and mutations in this gene have been associated with an infantile multisystem neurologic, endocrine, and pancreatic disease (INMEPD) characterized by intellectual disability, postnatal microcephaly, progressive cerebellar atrophy, hearing impairment, polyneuropathy, failure to thrive, and organ fibrosis with exocrine pancreas insufficiency (PMID: 25574476). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]
UniProt Code:	Q9Y3E5
NCBI GenInfo Identifier:	6686183
NCBI Gene ID:	51651
NCBI Accession:	Q9Y3E5.1
UniProt Secondary Accession:	Q9Y3E5,Q9NTE5, B3KUY4,
UniProt Related Accession:	Q9Y3E5
Molecular Weight:	179
NCBI Full Name:	Peptidyl-tRNA hydrolase 2, mitochondrial
NCBI Synonym Full Names:	peptidyl-tRNA hydrolase 2
NCBI Official Symbol:	PTRH2
NCBI Official Synonym Symbols:	BIT1; PTH2; CFAP37; CGI-147
NCBI Protein Information:	peptidyl-tRNA hydrolase 2, mitochondrial; PTH 2; bcl-2 inhibitor of transcription 1; cilia and flagella associated protein 37
UniProt Protein Name:	Peptidyl-tRNA hydrolase 2, mitochondrial
UniProt Synonym Protein Names:	Bcl-2 inhibitor of transcription 1
UniProt Gene Name:	PTRH2
UniProt Entry Name:	PTH2_HUMAN

	Western blot analysis of extracts of various cell lines, using PTRH2 antibody (CAB6466) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using PTRH2 antibody (CAB6466) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spinal cord using PTRH2 antibody (CAB6466) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF-7 cells using PTRH2 antibody (CAB6466). Blue: DAPI for nuclear staining.