Antibody Name:	Anti-PTPRN2 Antibody
Antibody SKU:	CAB14774
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-242 of human PTPRN2 (NP_570858.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, SGC-7901, Mouse pancreas, Rat pancreas

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-242 of human PTPRN2 (NP_570858.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AAPS SVPR GRQL PGRL GCLL EEGL CGAS EACV NDGV FGRC QKVP AMDF YRYE VSPV ALQR LRVA LQKL SGTG FTWQ DDYT QYVM DQEL ADLP KTYL RRPE ASSP ARPS KHSV GSER RYSR EGGA ALAN ALRR HLPF LEAL SQAP ASDV LART HTAQ DRPP AEGD DRFS ESIL TYVA HTSA LTYP PGSR TQLR EDLL PRTL GQLQ PDEL SPKV DSGV DRHH L
Gene ID:	5799
Uniprot:	Q92932
Cellular Location:	Cytoplasmic vesicle, Single-pass type I membrane protein, secretory vesicle, secretory vesicle membrane, synaptic vesicle membrane
Calculated MW:	108kDa/109kDa/111kDa/114kDa
Observed MW:	111kDa

Synonyms:

PTPRN2, IA-2beta, IAR, ICAAR, PTPRP, R-PTP-N2

Background:

This gene encodes a protein with sequence similarity to receptor-like protein tyrosine phosphatases. However, tyrosine phosphatase activity has not been experimentally validated for this protein. Studies of the rat ortholog suggest that the encoded protein may instead function as a phosphatidylinositol phosphatase with the ability to dephosphorylate phosphatidylinositol 3-phosphate and phosphatidylinositol 4, 5-diphosphate, and this function may be involved in the regulation of insulin secretion. This protein has been identified as an autoantigen in insulin-dependent diabetes mellitus. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	PTPRN2: Implicated in development of nervous system and pancreatic endocrine cells. Belongs to the protein-tyrosine phosphatase family. Receptor class 8 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; EC 3.1.3.48; Membrane protein, integral; Receptor protein phosphatase, tyrosine Chromosomal Location of Human Ortholog: 7q36 Cellular Component: integral to plasma membrane; receptor complex; secretory granule membrane; synaptic vesicle membrane Molecular Function:transmembrane receptor protein tyrosine phosphatase activity Biological Process: neurotransmitter secretion; protein amino acid dephosphorylation
NCBI Summary:	This gene encodes a protein with sequence similarity to receptor-like protein tyrosine phosphatases. However, tyrosine phosphatase activity has not been experimentally validated for this protein. Studies of the rat ortholog suggest that the encoded protein may instead function as a phosphatidylinositol phosphatase with the ability to dephosphorylate phosphatidylinositol 3-phosphate and phosphatidylinositol 4,5-diphosphate, and this function may be involved in the regulation of insulin secretion. This protein has been identified as an autoantigen in insulin-dependent diabetes mellitus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]
UniProt Code:	Q92932
NCBI GenInfo Identifier:	116242738
NCBI Gene ID:	5799
NCBI Accession:	Q92932.2
UniProt Secondary Accession:	Q92932,Q8N4I5, Q92662, Q9Y4F8, Q9Y4I6, E9PC57,
UniProt Related Accession:	Q92932
Molecular Weight:	109,622 Da
NCBI Full Name:	Receptor-type tyrosine-protein phosphatase N2
NCBI Synonym Full Names:	protein tyrosine phosphatase, receptor type N2
NCBI Official Symbol:	PTPRN2
NCBI Official Synonym Symbols:	IAR; ICAAR; PTPRP; IA-2beta; R-PTP-N2
NCBI Protein Information:	receptor-type tyrosine-protein phosphatase N2
UniProt Protein Name:	Receptor-type tyrosine-protein phosphatase N2
UniProt Synonym Protein Names:	Islet cell autoantigen-related protein
Protein Family:	Receptor-type tyrosine-protein phosphatase
UniProt Gene Name:	PTPRN2
UniProt Entry Name:	PTPR2_HUMAN

	Western blot analysis of extracts of various cell lines, using PTPRN2 antibody (CAB14774) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded human mammary cancer using PTPRN2 antibody (CAB14774) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using PTPRN2 antibody (CAB14774) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using PTPRN2 antibody (CAB14774) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse pancreas using PTPRN2 antibody (CAB14774) at dilution of 1:100 (40x lens).