Antibody Name:	Anti-PTPN3 Antibody
Antibody SKU:	CAB6400
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PTPN3 (NP_001138840.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, BT-474, 293T, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PTPN3 (NP_001138840.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTSR LRAL GGRI NNIR TSEL PKEK TRSE VICS IHFL DGVV QTFK VTKQ DTGQ VLLD MVHN HLGV TEKE YFGL QHDD DSVD SPRW LEAS KAIR KQLK GGFP CTLH FRVR FFIP DPNT LQQE QTRH LYFL QLKM DICE GRLT CPLN SAVV LASY AVQS HFGD YNSS IHHP GYLS DSHF IPDQ NEDF LTKV ESLH EQHS GLKQ SEAE SCYI NIAR TLDF YGVE LHSG RDLH NLDL MIGI ASAG VAVY RKYI CTSF YPWV NILK ISFK RKKF FIHQ RQKQ AESR EHIV AFNM LNYR SCKN LWKS
Gene ID:	5774
Uniprot:	P26045
Cellular Location:	Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, cytoskeleton
Calculated MW:	83kDa/88kDa/103kDa
Observed MW:	103kDa

Synonyms:

PTPN3, PTP-H1, PTPH1

Background:

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This protein contains a C-terminal PTP domain and an N-terminal domain homologous to the band 4.1 superfamily of cytoskeletal-associated proteins. P97, a cell cycle regulator involved in a variety of membrane related functions, has been shown to be a substrate of this PTP. This PTP was also found to interact with, and be regulated by adaptor protein 14-3-3 beta. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	PTPN3: May act at junctions between the membrane and the cytoskeleton. Possesses tyrosine phosphatase activity. Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.
UniProt Protein Details:	Protein type:EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, tyrosine (non-receptor) Chromosomal Location of Human Ortholog: 9q31 Cellular Component: internal side of plasma membrane; cytoskeleton; cytoplasm Molecular Function:protein binding; sodium channel regulator activity; phosphotyrosine binding; cytoskeletal protein binding; protein tyrosine phosphatase activity; ATPase binding Biological Process: negative regulation of mitotic cell cycle; protein amino acid dephosphorylation; negative regulation of membrane protein ectodomain proteolysis
NCBI Summary:	The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This protein contains a C-terminal PTP domain and an N-terminal domain homologous to the band 4.1 superfamily of cytoskeletal-associated proteins. P97, a cell cycle regulator involved in a variety of membrane related functions, has been shown to be a substrate of this PTP. This PTP was also found to interact with, and be regulated by adaptor protein 14-3-3 beta. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]
UniProt Code:	P26045
NCBI GenInfo Identifier:	229462761
NCBI Gene ID:	5774
NCBI Accession:	P26045.2
UniProt Secondary Accession:	P26045,A0AUW9, E7EN99, E9PGU7,
UniProt Related Accession:	P26045
Molecular Weight:	913
NCBI Full Name:	Tyrosine-protein phosphatase non-receptor type 3
NCBI Synonym Full Names:	protein tyrosine phosphatase, non-receptor type 3
NCBI Official Symbol:	PTPN3
NCBI Official Synonym Symbols:	PTPH1; PTP-H1
NCBI Protein Information:	tyrosine-protein phosphatase non-receptor type 3; protein-tyrosine phosphatase H1; cytoskeletal-associated protein tyrosine phosphatase
UniProt Protein Name:	Tyrosine-protein phosphatase non-receptor type 3
UniProt Synonym Protein Names:	Protein-tyrosine phosphatase H1; PTP-H1
Protein Family:	Tyrosine-protein phosphatase non-receptor
UniProt Gene Name:	PTPN3
UniProt Entry Name:	PTN3_HUMAN

	Western blot analysis of extracts of various cell lines, using PTPN3 antibody (CAB6400) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat brain using PTPN3 antibody (CAB6400) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using PTPN3 antibody (CAB6400) at dilution of 1:100 (40x lens).