Cell Biology Antibodies 8

Anti-PTPN11 Antibody (CAB2793)

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SKU:
CAB2793
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PTPN11 Antibody
Antibody SKU:CAB2793
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 514-593 of human PTPN11 (NP_002825.3).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, Jurkat, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 514-593 of human PTPN11 (NP_002825.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IYMA VQHY IETL QRRI EEEQ KSKR KGHE YTNI KYSL ADQT SGDQ SPLP PCTP TPPC AEMR EDSA RVYE NVGL MQQQ KSFR
Gene ID:5781
Uniprot:Q06124
Cellular Location:Cytoplasm
Calculated MW:52kDa/68kDa
Observed MW:68kDa
Synonyms:BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2, PTPN11
Background:The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:SHP-2: a SH2-containing a ubiquitously expressed tyrosine-specific protein phosphatase. It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens and extracellular matrices in the control of cell growth, differentiation, migration, and death. Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Protein phosphatase, tyrosine (non-receptor); EC 3.1.3.48

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function:1-phosphatidylinositol-3-kinase activity; insulin receptor binding; non-membrane spanning protein tyrosine phosphatase activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphoprotein phosphatase activity; protein binding; protein tyrosine phosphatase activity; SH3/SH2 adaptor activity

Biological Process: brain development; ephrin receptor signaling pathway; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; genitalia development; heart development; inner ear development; leukocyte migration; phosphoinositide-mediated signaling; platelet activation; regulation of cell adhesion mediated by integrin; regulation of phosphoinositide 3-kinase cascade; regulation of protein complex assembly; T cell costimulation

Disease: Juvenile Myelomonocytic Leukemia; Leopard Syndrome 1; Metachondromatosis; Noonan Syndrome 1

NCBI Summary:The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
UniProt Code:Q06124
NCBI GenInfo Identifier:84028248
NCBI Gene ID:5781
NCBI Accession:Q06124.2
UniProt Secondary Accession:Q06124,Q96HD7, A8K1D9,
UniProt Related Accession:Q06124
Molecular Weight:52,828 Da
NCBI Full Name:Tyrosine-protein phosphatase non-receptor type 11
NCBI Synonym Full Names:protein tyrosine phosphatase, non-receptor type 11
NCBI Official Symbol:PTPN11  
NCBI Official Synonym Symbols:CFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3  
NCBI Protein Information:tyrosine-protein phosphatase non-receptor type 11
UniProt Protein Name:Tyrosine-protein phosphatase non-receptor type 11
UniProt Synonym Protein Names:Protein-tyrosine phosphatase 1D; PTP-1D; Protein-tyrosine phosphatase 2C; PTP-2C; SH-PTP2; SHP-2; Shp2; SH-PTP3
Protein Family:Tyrosine-protein phosphatase non-receptor
UniProt Gene Name:PTPN11  
UniProt Entry Name:PTN11_HUMAN
Anti-PTPN11 Antibody (CAB2793)Western blot analysis of extracts of various cell lines, using PTPN11 antibody (CAB2793) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-PTPN11 Antibody (CAB2793)Immunohistochemistry of paraffin-embedded rat heart using PTPN11 antibody (CAB2793) at dilution of 1:100 (40x lens).
Anti-PTPN11 Antibody (CAB2793)Immunohistochemistry of paraffin-embedded human gastric cancer using PTPN11 antibody (CAB2793) at dilution of 1:100 (40x lens).
Anti-PTPN11 Antibody (CAB2793)Immunohistochemistry of paraffin-embedded mouse spleen using PTPN11 antibody (CAB2793) at dilution of 1:100 (40x lens).
Anti-PTPN11 Antibody (CAB2793)Immunofluorescence analysis of U2OS cells using PTPN11 antibody (CAB2793) at dilution of 1:100. Blue: DAPI for nuclear staining.
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