KO Validated Antibodies 1

Anti-PTPN11 Antibody (CAB12486)[KO Validated]

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SKU:
CAB12486
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PTPN11 Antibody [KO Validated]
Antibody SKU:CAB12486
Antibody Size:20uL, 50uL, 100uL
Application:WB IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-460 of human PTPN11 (NP_002825.3).
Application:WB IF IP
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, Jurkat, A-431, MCF-7, Mouse brain, Rat brain, Rat lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-460 of human PTPN11 (NP_002825.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTSR RWFH PNIT GVEA ENLL LTRG VDGS FLAR PSKS NPGD FTLS VRRN GAVT HIKI QNTG DYYD LYGG EKFA TLAE LVQY YMEH HGQL KEKN GDVI ELKY PLNC ADPT SERW FHGH LSGK EAEK LLTE KGKH GSFL VRES QSHP GDFV LSVR TGDD KGES NDGK SKVT HVMI RCQE LKYD VGGG ERFD SLTD LVEH YKKN PMVE TLGT VLQL KQPL NTTR INAA EIES RVRE LSKL AETT DKVK QGFW EEFE TLQQ QECK LLYS RKEG QRQE NKNK NRYK NILP FDHT RVVL HDGD PNEP VSDY INAN IIMP EFET KCNN SKPK KSYI ATQG CLQN TVND FWRM VFQE NSRV IVMT TKEV ERGK SKCV KYWP DEYA LKEY GVMR VRNV KESA AHDY TLRE LKLS KVGQ GNTE RTVW QYHF RTWP DHGV PSDP GGVL DFLE EVHH KQES IMDA GPVV VHCS
Gene ID:5781
Uniprot:Q06124
Cellular Location:Cytoplasm
Calculated MW:52kDa/68kDa
Observed MW:75kDa
Synonyms:BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2, PTPN11
Background:The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:SHP-2: a SH2-containing a ubiquitously expressed tyrosine-specific protein phosphatase. It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens and extracellular matrices in the control of cell growth, differentiation, migration, and death. Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Protein phosphatase, tyrosine (non-receptor); EC 3.1.3.48

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function:1-phosphatidylinositol-3-kinase activity; insulin receptor binding; non-membrane spanning protein tyrosine phosphatase activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphoprotein phosphatase activity; protein binding; protein tyrosine phosphatase activity; SH3/SH2 adaptor activity

Biological Process: brain development; ephrin receptor signaling pathway; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; genitalia development; heart development; inner ear development; leukocyte migration; phosphoinositide-mediated signaling; platelet activation; regulation of cell adhesion mediated by integrin; regulation of phosphoinositide 3-kinase cascade; regulation of protein complex assembly; T cell costimulation

Disease: Juvenile Myelomonocytic Leukemia; Leopard Syndrome 1; Metachondromatosis; Noonan Syndrome 1

NCBI Summary:The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
UniProt Code:Q06124
NCBI GenInfo Identifier:84028248
NCBI Gene ID:5781
NCBI Accession:Q06124.2
UniProt Secondary Accession:Q06124,Q96HD7, A8K1D9,
UniProt Related Accession:Q06124
Molecular Weight:52,828 Da
NCBI Full Name:Tyrosine-protein phosphatase non-receptor type 11
NCBI Synonym Full Names:protein tyrosine phosphatase, non-receptor type 11
NCBI Official Symbol:PTPN11  
NCBI Official Synonym Symbols:CFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3  
NCBI Protein Information:tyrosine-protein phosphatase non-receptor type 11
UniProt Protein Name:Tyrosine-protein phosphatase non-receptor type 11
UniProt Synonym Protein Names:Protein-tyrosine phosphatase 1D; PTP-1D; Protein-tyrosine phosphatase 2C; PTP-2C; SH-PTP2; SHP-2; Shp2; SH-PTP3
Protein Family:Tyrosine-protein phosphatase non-receptor
UniProt Gene Name:PTPN11  
UniProt Entry Name:PTN11_HUMAN
Anti-PTPN11 Antibody [KO Validated] (CAB12486)Western blot analysis of extracts from normal (control) and PTPN11 knockout (KO) 293T cells, using PTPN11 antibody (CAB12486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-PTPN11 Antibody [KO Validated] (CAB12486)Western blot analysis of extracts of various cell lines, using PTPN11 antibody (CAB12486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-PTPN11 Antibody [KO Validated] (CAB12486)Immunofluorescence analysis of C6 cells using PTPN11 antibody (CAB12486) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-PTPN11 Antibody [KO Validated] (CAB12486)Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug PTPN11 antibody (CAB12486). Western blot was performed from the immunoprecipitate using PTPN11 antibody (CAB12486) at a dilition of 1:2000.
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