Antibody Name:	Anti-PTPN1 Antibody [KO Validated]
Antibody SKU:	CAB1590
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 281-435 of human PTPN1 (NP_002818.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, SKOV3, U-251MG, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 281-435 of human PTPN1 (NP_002818.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IMGD SSVQ DQWK ELSH EDLE PPPE HIPP PPRP PKRI LEPH NGKC REFF PNHQ WVKE ETQE DKDC PIKE EKGS PLNA APYG IESM SQDT EVRS RVVG GSLR GAQA ASPA KGEP SLPE KDED HALS YWKP FLVN MCVA TVLT AGAY LCYR FLFN SNT
Gene ID:	5770
Uniprot:	P18031
Cellular Location:	Cytoplasmic side, Endoplasmic reticulum membrane, Peripheral membrane protein
Calculated MW:	49kDa
Observed MW:	50kDa

Synonyms:

PTPN1, PTP1B

Background:

The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	PTP1B: a non-receptor phospho-tyrosine protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation and inactivation of PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. Negatively regulates insulin signaling by dephosphorylating the phosphotyrosine residues of insulin receptor. Also reported to dephosphorylate integrin, epidermal growth factor receptor, JAK2 and TYK2, regulating cell growth control and the cellular response to interferon. May regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET. PTP1B knockout mice show resistance to dietary weight gain and enhanced insulin sensitivity, suggesting a possible role in treatment of obesity as well as type 2 diabetes.
UniProt Protein Details:	Protein type:EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor); Motility/polarity/chemotaxis; Phosphatase Chromosomal Location of Human Ortholog: 20q13.1-q13.2 Cellular Component: endoplasmic reticulum; early endosome; plasma membrane; cytoplasmic vesicle; cytosol Molecular Function:protein binding; enzyme binding; ephrin receptor binding; zinc ion binding; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; protein kinase binding; insulin receptor binding Biological Process: platelet activation; unfolded protein response, activation of signaling protein activity; negative regulation of vascular endothelial growth factor receptor signaling pathway; unfolded protein response; cytokine and chemokine mediated signaling pathway; actin cytoskeleton reorganization; regulation of endocytosis; insulin receptor signaling pathway; regulation of signal transduction; negative regulation of insulin receptor signaling pathway; blood coagulation Disease: Diabetes Mellitus, Noninsulin-dependent
NCBI Summary:	The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]
UniProt Code:	P18031
NCBI GenInfo Identifier:	131467
NCBI Gene ID:	5770
NCBI Accession:	P18031.1
UniProt Secondary Accession:	P18031,Q5TGD8, Q9BQV9, Q9NQQ4,
UniProt Related Accession:	P18031
Molecular Weight:	49,967 Da
NCBI Full Name:	Tyrosine-protein phosphatase non-receptor type 1
NCBI Synonym Full Names:	protein tyrosine phosphatase, non-receptor type 1
NCBI Official Symbol:	PTPN1
NCBI Official Synonym Symbols:	PTP1B
NCBI Protein Information:	tyrosine-protein phosphatase non-receptor type 1; protein-tyrosine phosphatase 1B; protein tyrosine phosphatase, placental
UniProt Protein Name:	Tyrosine-protein phosphatase non-receptor type 1
UniProt Synonym Protein Names:	Protein-tyrosine phosphatase 1B; PTP-1B
Protein Family:	Tyrosine-protein phosphatase non-receptor
UniProt Gene Name:	PTPN1
UniProt Entry Name:	PTN1_HUMAN

	Western blot analysis of extracts of various cell lines, using PTPN1 antibody (CAB1590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts from normal (control) and PTPN1 knockout (KO) HeLa cells, using PTPN1 antibody (CAB1590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat brain using PTPN1 antibody (CAB1590) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using PTPN1 antibody (CAB1590) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF-7 cells using PTPN1 antibody (CAB1590). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
PTPN1 Antibody (PACO01376)	Human PTP1B ELISA Kit
Phospho-PTPN1 (S50) Antibody (PACO03046)	Human Tyrosine-protein phosphatase non-receptor type 1 (PTPN1) ELISA Kit
PTPN1 Antibody (PACO11585)	PTP1B Colorimetric Cell-Based ELISA Kit
	PTP1B (Phospho-Ser50) Colorimetric Cell-Based ELISA Kit