Epigenetics & Nuclear Signaling Antibodies 2
Anti-PTCD1 Antibody (CAB16219)
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- SKU:
- CAB16219
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-PTCD1 Antibody |
| Antibody SKU: | CAB16219 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 435-537 of human PTCD1 (NP_056360.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, OVCAR3, A-549 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 435-537 of human PTCD1 (NP_056360.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PVEL EVNL LTPG AVPP TVVS FGTV TTPA DRLA LIGG LEGF LSKM AEHR QQPD IRTL TLLA EVVE SGSP AESL LLAL LDEH QVEA DLTF FNTL VRKK SKLG DLE |
| Gene ID: | 26024 |
| Uniprot: | O75127 |
| Cellular Location: | |
| Calculated MW: | 78kDa |
| Observed MW: | 79kDa |
| Synonyms: | PTCD1 |
| Background: | This gene encodes a mitochondrial protein that binds leucine tRNAs and other mitochondrial RNAs and plays a role in the regulation of translation. Increased expression of this gene results in decreased mitochondrial leucine tRNA levels. Naturally occurring read-through transcription exists between upstream ATP5J2 (ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F2) and this gene. |
| NCBI Summary: | This gene encodes a mitochondrial protein that binds leucine tRNAs and other mitochondrial RNAs and plays a role in the regulation of translation. Increased expression of this gene results in decreased mitochondrial leucine tRNA levels. Naturally occurring read-through transcription exists between upstream ATP5J2 (ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F2) and this gene. [provided by RefSeq, Aug 2015] |
| UniProt Code: | O75127 |
| NCBI GenInfo Identifier: | 38492358 |
| NCBI Gene ID: | 26024 |
| NCBI Accession: | NP_056360 |
| UniProt Related Accession: | O75127 |
| Molecular Weight: | 77kDa |
| NCBI Full Name: | pentatricopeptide repeat-containing protein 1, mitochondrial |
| NCBI Synonym Full Names: | pentatricopeptide repeat domain 1 |
| NCBI Official Symbol: | PTCD1 |
| NCBI Protein Information: | pentatricopeptide repeat-containing protein 1, mitochondrial |
| UniProt Protein Name: | Pentatricopeptide repeat-containing protein 1, mitochondrial |
| Protein Family: | Pentatricopeptide repeat-containing protein |
| UniProt Gene Name: | PTCD1 |
| UniProt Entry Name: | PTCD1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PTCD1 antibody (CAB16219) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of C6 cells using PTCD1 antibody (CAB16219) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using PTCD1 antibody (CAB16219) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using PTCD1 antibody (CAB16219) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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