Antibody Name:	Anti-PSTPIP1 Antibody
Antibody SKU:	CAB7760
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 157-416 of human PSTPIP1 (NP_003969.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	THP-1, mouse spleen, mouse thymus, mouse brain, rat brain, Mouse spleen, Mouse thymus, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 157-416 of human PSTPIP1 (NP_003969.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AFER ISAN GHQK QVEK SQNK ARQC KDSA TEAE RVYR QSIA QLEK VRAE WEQE HRTT CEAF QLQE FDRL TILR NALW VHSN QLSM QCVK DDEL YEEV RLTL EGCS IDAD IDSF IQAK STGT EPPA PVPY QNYY DREV TPLT SSPG IQPS CGMI KRFS GLLH GSPK TTSL AASA ASTE TLTP TPER NEGV YTAI AVQE IQGN PASP AQEY RALY DYTA QNPD ELDL SAGD ILEV ILEG EDGW WTVE RNGQ RGFV PGSY LEKL
Gene ID:	9051
Uniprot:	O43586
Cellular Location:	Cell projection, Cleavage furrow, Cytoplasm, cytoskeleton, lamellipodium, perinuclear region, uropodium
Calculated MW:	45kDa/47kDa
Observed MW:	48kDa

Synonyms:

PSTPIP1, CD2BP1, CD2BP1L, CD2BP1S, H-PIP, PAPAS, PSTPIP

Background:

This gene encodes a cytoskeletal protein that is highly expressed in hemopoietic tissues. This protein functions via its interaction with several different proteins involved in cytoskeletal organization and inflammatory processes. It binds to the cytoplasmic tail of CD2, an effector of T cell activation and adhesion, downregulating CD2-triggered adhesion. It binds PEST-type protein tyrosine phosphatases (PTP) and directs them to c-Abl kinase to mediate c-Abl dephosphorylation, thereby, regulating c-Abl activity. It also interacts with pyrin, which is found in association with the cytoskeleton in myeloid/monocytic cells and modulates immunoregulatory functions. Mutations in this gene are associated with PAPA (pyogenic sterile arthritis, pyoderma gangrenosum, and acne) syndrome. It is hypothesized that the disease-causing mutations compromise physiologic signaling necessary for the maintenance of a proper inflammatory response.

UniProt Protein Function:	PSTPIP1: an SH3-domain containing protein that may play a role in vesicle formation and transport. Interacts with PTP-PEST to inhibit WASp-driven actin polymerization and synapse formation.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Adaptor/scaffold Chromosomal Location of Human Ortholog: 15q24.3 Cellular Component: membrane; lamellipodium; perinuclear region of cytoplasm; contractile ring; stress fiber; uropod; cytosol; cleavage furrow Molecular Function:protein binding; actin binding; protein phosphatase binding Biological Process: innate immune response; endocytosis; inflammatory response; signal transduction; cell adhesion Disease: Pyogenic Sterile Arthritis, Pyoderma Gangrenosum, And Acne
NCBI Summary:	The protein encoded by this gene binds to the cytoplasmic tail of CD2, an effector of T cell activation and adhesion, negatively affecting CD2-triggered T cell activation. The encoded protein appears to be a scaffold protein and a regulator of the actin cytoskeleton. It has also been shown to bind ABL1, PTPN18, WAS, CD2AP, and PTPN12. Mutations in this gene are a cause of PAPA syndrome. [provided by RefSeq, Jul 2008]
UniProt Code:	O43586
NCBI GenInfo Identifier:	74739557
NCBI Gene ID:	9051
NCBI Accession:	O43586.1
UniProt Secondary Accession:	O43586,O43585, O95657, B5BU74, B5BUK4,
UniProt Related Accession:	O43586
Molecular Weight:	416
NCBI Full Name:	Proline-serine-threonine phosphatase-interacting protein 1
NCBI Synonym Full Names:	proline-serine-threonine phosphatase interacting protein 1
NCBI Official Symbol:	PSTPIP1
NCBI Official Synonym Symbols:	H-PIP; PAPAS; CD2BP1; PSTPIP; CD2BP1L; CD2BP1S
NCBI Protein Information:	proline-serine-threonine phosphatase-interacting protein 1; CD2-binding protein 1; CD2 antigen-binding protein 1; CD2 cytoplasmic tail-binding protein; PEST phosphatase-interacting protein 1
UniProt Protein Name:	Proline-serine-threonine phosphatase-interacting protein 1
UniProt Synonym Protein Names:	CD2-binding protein 1; H-PIP
Protein Family:	Proline-serine-threonine phosphatase-interacting protein
UniProt Gene Name:	PSTPIP1
UniProt Entry Name:	PPIP1_HUMAN

	Western blot analysis of extracts of various cell lines, using PSTPIP1 antibody (CAB7760) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 40s.
	Immunohistochemistry of paraffin-embedded human stomach using PSTPIP1 antibody (CAB7760) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using PSTPIP1 antibody (CAB7760) at dilution of 1:100 (40x lens).