Cell Biology Antibodies 12

Anti-PSPC1 Antibody (CAB9209)

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SKU:
CAB9209
Product Type:
Antibody
Applications:
WB
Applications:
IP
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PSPC1 Antibody
Antibody SKU:CAB9209
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human PSPC1 (NP_001035879.1).
Application:WB IHC IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:BxPC-3, A-549, U-87MG, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human PSPC1 (NP_001035879.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MMLR GNLK QVRI EKNP ARLR ALES AVGE SEPA AAAA MALA LAGE PAPP APAP PEDH PDEE MGFT IDIK SF
Gene ID:55269
Uniprot:Q8WXF1
Cellular Location:Cytoplasm, Nucleus, Nucleus matrix, Nucleus speckle, nucleolus
Calculated MW:45kDa/58kDa
Observed MW:65kDa
Synonyms:PSPC1, PSP1
Background:This gene encodes a nucleolar protein that localizes to punctate subnuclear structures that occur close to splicing speckles, known as paraspeckles. These paraspeckles are composed of RNA-protein structures that include a non-coding RNA, NEAT1/Men epsilon/beta, and the Drosophila Behavior Human Splicing family of proteins, which include the product of this gene and the P54NRB/NONO and PSF/SFPQ proteins. Paraspeckles may function in the control of gene expression via an RNA nuclear retention mechanism. The protein encoded by this gene is found in paraspeckles in transcriptionally active cells, but it localizes to unique cap structures at the nucleolar periphery when RNA polymerase II transcription is inhibited, or during telophase. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene, which is also located on chromosome 13, has been identified.
UniProt Protein Function:PSPC1: Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line. Binds to poly(A), poly(G) and poly(U) RNA homopolymers. Together with NONO, required for the formation of nuclear paraspeckles. Belongs to the PSPC family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:RNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 13q12.11

Cellular Component: nucleoplasm; nuclear matrix; cytoplasm; nucleolus; nuclear speck; paraspeckles

Molecular Function:protein binding; nucleotide binding

Biological Process: transcription, DNA-dependent; rhythmic process; negative regulation of transcription, DNA-dependent; regulation of circadian rhythm

NCBI Summary:This gene encodes a nucleolar protein that localizes to punctate subnuclear structures that occur close to splicing speckles, known as paraspeckles. These paraspeckles are composed of RNA-protein structures that include a non-coding RNA, NEAT1/Men epsilon/beta, and the Drosophila Behavior Human Splicing family of proteins, which include the product of this gene and the P54NRB/NONO and PSF/SFPQ proteins. Paraspeckles may function in the control of gene expression via an RNA nuclear retention mechanism. The protein encoded by this gene is found in paraspeckles in transcriptionally active cells, but it localizes to unique cap structures at the nucleolar periphery when RNA polymerase II transcription is inhibited, or during telophase. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene, which is also located on chromosome 13, has been identified. [provided by RefSeq, Aug 2011]
UniProt Code:Q8WXF1
NCBI GenInfo Identifier:109240550
NCBI Gene ID:55269
NCBI Accession:NP_001035879.1
UniProt Secondary Accession:Q8WXF1,Q5JTQ3, Q8NCZ9, Q8WXE8, Q9NV36,
UniProt Related Accession:Q8WXF1
Molecular Weight:58,744 Da
NCBI Full Name:paraspeckle component 1
NCBI Synonym Full Names:paraspeckle component 1
NCBI Official Symbol:PSPC1  
NCBI Official Synonym Symbols:PSP1  
NCBI Protein Information:paraspeckle component 1; paraspeckle protein 1
UniProt Protein Name:Paraspeckle component 1
UniProt Synonym Protein Names:Paraspeckle protein 1
Protein Family:Paraspeckle component
UniProt Gene Name:PSPC1  
UniProt Entry Name:PSPC1_HUMAN
Anti-PSPC1 Polyclonal Antibody (CAB9209)Western blot analysis of extracts of various cell lines, using PSPC1 antibody (CAB9209) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-PSPC1 Polyclonal Antibody (CAB9209)Immunohistochemistry of paraffin-embedded Mouse testis using PSPC1 Rabbit pAb (CAB9209) at dilution of 1:100 (40x lens).
Anti-PSPC1 Polyclonal Antibody (CAB9209)Immunohistochemistry of paraffin-embedded Rat brain using PSPC1 Rabbit pAb (CAB9209) at dilution of 1:100 (40x lens).
Anti-PSPC1 Polyclonal Antibody (CAB9209)Immunohistochemistry of paraffin-embedded Human esophageal using PSPC1 Rabbit pAb (CAB9209) at dilution of 1:100 (40x lens).
Anti-PSPC1 Polyclonal Antibody (CAB9209)Immunoprecipitation analysis of 200ug extracts of A-549 cells using 3ug PSPC1 antibody (CAB9209). Western blot was performed from the immunoprecipitate using PSPC1 antibody (CAB9209) at a dilition of 1:1000.
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