KO Validated Antibodies 2

Anti-PSME4 Antibody (CAB19873)[KO Validated]

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SKU:
CAB19873
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Developmental Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-PSME4 Antibody [KO Validated]
Antibody SKU:CAB19873
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human PSME4.
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant protein of human PSME4.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:23198
Uniprot:Q14997
Cellular Location:Cytoplasm, Nucleus, Nucleus speckle, cytosol
Calculated MW:24kDa/113kDa/139kDa/211kDa
Observed MW:211KDa
Anti-PSME4 Antibody [KO Validated] (CAB19873)Western blot analysis of extracts from normal (control) and PSME4 knockout (KO) HeLa cells, using PSME4 antibody (CAB19873) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:PSME4, PA200
Background:
UniProt Protein Function:Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks.
UniProt Code:Q14997
NCBI GenInfo Identifier:163644283
NCBI Gene ID:23198
NCBI Accession:NP_055429.2
UniProt Secondary Accession:Q14997,Q1XBG4, Q1XBG5, Q1XBG6, Q2M1Z0, Q6IPR2, Q86XF8
UniProt Related Accession:Q14997
Molecular Weight:Calculated: 24kDa; 113kDa; 139kDa; 211kDaObserved: 211kDa
NCBI Full Name:proteasome activator complex subunit 4
NCBI Synonym Full Names:proteasome activator subunit 4
NCBI Official Symbol:PSME4  
NCBI Official Synonym Symbols:PA200  
NCBI Protein Information:proteasome activator complex subunit 4
UniProt Protein Name:Proteasome activator complex subunit 4
UniProt Synonym Protein Names:Proteasome activator PA200
Protein Family:Proteasome activator complex
UniProt Gene Name:PSME4  
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