Antibody Name:	Anti-PSMD8 Antibody
Antibody SKU:	CAB6955
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 101-350 of human PSMD8 (NP_002803.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	SGC-7901, Jurkat, SW480, U-87MG, Mouse testis, Mouse skeletal muscle, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 101-350 of human PSMD8 (NP_002803.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EWNR KSPN LSKC GEEL GRLK LVLL ELNF LPTT GTKL TKQQ LILA RDIL EIGA QWSI LRKD IPSF ERYM AQLK CYYF DYKE QLPE SAYM HQLL GLNL LFLL SQNR VAEF HTEL ERLP AKDI QTNV YIKH PVSL EQYL MEGS YNKV FLAK GNIP AESY TFFI DILL DTIR DEIA GCIE KAYE KILF TEAT RILF FNTP KKMT DYAK KRGW VLGP NNYY SFAS QQQK PEDT TIPS TELA KQVI EYAR QLEM IV
Gene ID:	5714
Uniprot:	P48556
Cellular Location:
Calculated MW:	39kDa
Observed MW:	30-35kDa

Synonyms:

PSMD8, HEL-S-91n, HIP6, HYPF, Nin1p, Rpn12, S14, p31

Background:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 1.

UniProt Protein Function:	Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.
NCBI Summary:	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 1. [provided by RefSeq, Jul 2008]
UniProt Code:	P48556
NCBI GenInfo Identifier:	308153477
NCBI Gene ID:	5714
NCBI Accession:	P48556.2
UniProt Related Accession:	P48556
Molecular Weight:	~ 30-35kDa
NCBI Full Name:	26S proteasome non-ATPase regulatory subunit 8
NCBI Synonym Full Names:	proteasome 26S subunit, non-ATPase 8
NCBI Official Symbol:	PSMD8
NCBI Official Synonym Symbols:	S14; p31; HIP6; HYPF; Nin1p; Rpn12; HEL-S-91n
NCBI Protein Information:	26S proteasome non-ATPase regulatory subunit 8
UniProt Protein Name:	26S proteasome non-ATPase regulatory subunit 8
UniProt Synonym Protein Names:	26S proteasome regulatory subunit RPN12; 26S proteasome regulatory subunit S14; p31
Protein Family:	26S proteasome non-ATPase regulatory
UniProt Gene Name:	PSMD8

	Western blot analysis of extracts of various cell lines, using PSMD8 antibody (CAB6955) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver damage using PSMD8 antibody (CAB6955) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using PSMD8 antibody (CAB6955).