Antibody Name:	Anti-PSMD12 Antibody
Antibody SKU:	CAB6708
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 197-456 of human PSMD12 (NP_002807.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, BT-474, HT-29, Mouse heart, Rat heart, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 197-456 of human PSMD12 (NP_002807.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KDYI RTQI ISKK INTK FFQE ENTE KLKL KYYN LMIQ LDQH EGSY LSIC KHYR AIYD TPCI QAES EKWQ QALK SVVL YVIL APFD NEQS DLVH RISG DKKL EEIP KYKD LLKL FTTM ELMR WSTL VEDY GMEL RKGS LESP ATDV FGST EEGE KRWK DLKN RVVE HNIR IMAK YYTR ITMK RMAQ LLDL SVDE SEAF LSNL VVNK TIFA KVDR LAGI INFQ RPKD PNNL LNDW SQKL NSLM SLVN KTTH LIAK EEMI HNLQ
Gene ID:	5718
Uniprot:	O00232
Cellular Location:
Calculated MW:	50kDa/52kDa
Observed MW:	53kDa

Synonyms:

PSMD12, Rpn5, p55, STISS

Background:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	PSMD12: Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Belongs to the proteasome subunit p55 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Protease; Proteasome complex Chromosomal Location of Human Ortholog: 17q24.2 Cellular Component: proteasome complex; nucleoplasm; membrane; proteasome regulatory particle; cytosol Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis
NCBI Summary:	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 3. [provided by RefSeq, Jul 2008]
UniProt Code:	O00232
NCBI GenInfo Identifier:	20978544
NCBI Gene ID:	5718
NCBI Accession:	O00232.3
UniProt Secondary Accession:	O00232,Q53HA2, Q6P053, A6NP15,
UniProt Related Accession:	O00232
Molecular Weight:	50,579 Da
NCBI Full Name:	26S proteasome non-ATPase regulatory subunit 12
NCBI Synonym Full Names:	proteasome (prosome, macropain) 26S subunit, non-ATPase, 12
NCBI Official Symbol:	PSMD12
NCBI Official Synonym Symbols:	p55; Rpn5
NCBI Protein Information:	26S proteasome non-ATPase regulatory subunit 12; 26S proteasome regulatory subunit p55; 26S proteasome regulatory subunit RPN5
UniProt Protein Name:	26S proteasome non-ATPase regulatory subunit 12
UniProt Synonym Protein Names:	26S proteasome regulatory subunit RPN5; 26S proteasome regulatory subunit p55
Protein Family:	26S proteasome non-ATPase regulatory
UniProt Gene Name:	PSMD12
UniProt Entry Name:	PSD12_HUMAN

	Western blot analysis of extracts of various cell lines, using PSMD12 antibody (CAB6708) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human kidney using PSMD12 antibody (CAB6708) at dilution of 1:100 (40x lens).