Name: Anti-PSMD11 Antibody (CAB15306)
Brand: Cell Biology Antibodies 5
SKU: CAB15306
Availability: OutOfStock

Antibody Name:	Anti-PSMD11 Antibody
Antibody SKU:	CAB15306
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 253-422 of human PSMD11 (NP_002806.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	NCI-H460, 293T, Mouse brain, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 253-422 of human PSMD11 (NP_002806.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	YMLL CKIM LNTP EDVQ ALVS GKLA LRYA GRQT EALK CVAQ ASKN RSLA DFEK ALTD YRAE LRDD PIIS THLA KLYD NLLE QNLI RVIE PFSR VQIE HISS LIKL SKAD VERK LSQM ILDK KFHG ILDQ GEGV LIIF DEPP VDKT YEAA LETI QNMS KVVD SLYN KAKK LT
Gene ID:	5717
Uniprot:	O00231
Cellular Location:	Cytoplasm, Nucleus, cytosol
Calculated MW:	47kDa
Observed MW:	47KDa

Synonyms:

PSMD11, Rpn6, S9, p44.5

Background:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the proteasome subunit S9 family that functions as a non-ATPase subunit of the 19S regulator and is phosphorylated by AMP-activated protein kinase. Alternatively spliced transcript variants have been observed for this gene.

UniProt Protein Function:	PSMD11: Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity. Belongs to the proteasome subunit S9 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Protease; Proteasome complex Chromosomal Location of Human Ortholog: 17q11.2 Cellular Component: cytosol; membrane; nucleoplasm; nucleus; proteasome complex Molecular Function:protein binding Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; MAPKKK cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; proteasome assembly; protein polyubiquitination; regulation of amino acid metabolic process; regulation of mRNA stability; stem cell differentiation; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway; ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway, planar cell polarity pathway
NCBI Summary:	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the proteasome subunit S9 family that functions as a non-ATPase subunit of the 19S regulator and is phosphorylated by AMP-activated protein kinase. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]
UniProt Code:	O00231
NCBI GenInfo Identifier:	20978543
NCBI Gene ID:	5717
NCBI Accession:	O00231.3
UniProt Secondary Accession:	O00231,O00495, Q53FT5, A8K3I7, E1P663,
UniProt Related Accession:	O00231
Molecular Weight:	47,535 Da
NCBI Full Name:	26S proteasome non-ATPase regulatory subunit 11
NCBI Synonym Full Names:	proteasome 26S subunit, non-ATPase 11
NCBI Official Symbol:	PSMD11
NCBI Official Synonym Symbols:	S9; Rpn6; p44.5
NCBI Protein Information:	26S proteasome non-ATPase regulatory subunit 11
UniProt Protein Name:	26S proteasome non-ATPase regulatory subunit 11
UniProt Synonym Protein Names:	26S proteasome regulatory subunit RPN6; 26S proteasome regulatory subunit S9; 26S proteasome regulatory subunit p44.5
UniProt Gene Name:	PSMD11
UniProt Entry Name:	PSD11_HUMAN

Western blot analysis of extracts of various cell lines, using PSMD11 antibody (CAB15306) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies

PSMD11 Antibody (PACO01372)

PSMD11 Antibody (PACO11546)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)