Cell Death Antibodies 1

Anti-PSMD10 Antibody (CAB1949)

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SKU:
CAB1949
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death

Description

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Antibody Name:Anti-PSMD10 Antibody
Antibody SKU:CAB1949
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-226 of human PSMD10 (NP_002805.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SW620
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-226 of human PSMD10 (NP_002805.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEGC VSNL MVCN LAYS GKLE ELKE SILA DKSL ATRT DQDS RTAL HWAC SAGH TEIV EFLL QLGV PVND KDDA GWSP LHIA ASAG RDEI VKAL LGKG AQVN AVNQ NGCT PLHY AASK NRHE IAVM LLEG GANP DAKD HYEA TAMH RAAA KGNL KMIH ILLY YKAS TNIQ DTEG NTPL HLAC DEER VEEA KLLV SQGA SIYI ENKE EKTP LQVA KGGL GLIL KRMV EG
Gene ID:5716
Uniprot:O75832
Cellular Location:Cytoplasm, Nucleus
Calculated MW:16kDa/24kDa
Observed MW:24kDa
Synonyms:PSMD10, dJ889N15.2, p28, p28(GANK)
Background:This gene encodes a subunit of the PA700/19S complex, which is the regulatory component of the 26S proteasome. The 26S proteosome complex is required for ubiquitin-dependent protein degradation. This protein is a non-ATPase subunit that may be involved in protein-protein interactions. Aberrant expression of this gene may paly a role in tumorigenesis. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.
UniProt Protein Function:PSMD10: Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Proteasome complex; Protease

Chromosomal Location of Human Ortholog: Xq22.3

Cellular Component: proteasome complex; nucleoplasm; intermediate filament cytoskeleton; proteasome regulatory particle; cytoplasm; nucleus; cytosol

Molecular Function:protein binding; transcription factor binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of cyclin-dependent protein kinase activity; protein polyubiquitination; viral reproduction; apoptosis; negative regulation of transcription from RNA polymerase II promoter; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; negative regulation of DNA damage response, signal transduction by p53 class mediator; antigen processing and presentation of peptide antigen via MHC class I; cytoplasmic sequestering of NF-kappaB; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; negative regulation of MAPKKK cascade; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; positive regulation of cell growth; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of protein ubiquitination; inhibition of NF-kappaB transcription factor; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis

NCBI Summary:This gene encodes a subunit of the PA700/19S complex, which is the regulatory component of the 26S proteasome. The 26S proteosome complex is required for ubiquitin-dependent protein degradation. This protein is a non-ATPase subunit that may be involved in protein-protein interactions. Aberrant expression of this gene may paly a role in tumorigenesis. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.[provided by RefSeq, Mar 2011]
UniProt Code:O75832
NCBI GenInfo Identifier:20532206
NCBI Gene ID:5716
NCBI Accession:O75832.1
UniProt Related Accession:O75832
Molecular Weight:16kDa; 24kDa
NCBI Full Name:26S proteasome non-ATPase regulatory subunit 10
NCBI Synonym Full Names:proteasome 26S subunit, non-ATPase 10
NCBI Official Symbol:PSMD10  
NCBI Official Synonym Symbols:p28; p28(GANK); dJ889N15.2  
NCBI Protein Information:26S proteasome non-ATPase regulatory subunit 10
UniProt Protein Name:26S proteasome non-ATPase regulatory subunit 10
UniProt Synonym Protein Names:26S proteasome regulatory subunit p28; Gankyrin; p28(GANK)
Protein Family:26S proteasome non-ATPase regulatory
UniProt Gene Name:PSMD10  
UniProt Entry Name:PSD10_HUMAN
Anti-PSMD10 Antibody (CAB1949)Western blot analysis of extracts of SW620 cells, using PSMD10 antibody (CAB1949) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-PSMD10 Antibody (CAB1949)Immunohistochemistry of paraffin-embedded rat brain using PSMD10 antibody (CAB1949) at dilution of 1:100 (40x lens).
Anti-PSMD10 Antibody (CAB1949)Immunohistochemistry of paraffin-embedded human breast cancer using PSMD10 antibody (CAB1949) at dilution of 1:100 (40x lens).
Anti-PSMD10 Antibody (CAB1949)Immunohistochemistry of paraffin-embedded mouse spleen using PSMD10 antibody (CAB1949) at dilution of 1:100 (40x lens).
Anti-PSMD10 Antibody (CAB1949)Immunofluorescence analysis of MCF7 cells using PSMD10 antibody (CAB1949). Blue: DAPI for nuclear staining.
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