Name: Anti-PSMD10 Antibody (CAB12469)
Brand: Cell Death Antibodies 1
SKU: CAB12469
Availability: OutOfStock

Antibody Name:	Anti-PSMD10 Antibody
Antibody SKU:	CAB12469
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 127-226 of human PSMD10 (NP_002805.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, HeLa, Mouse kidney, Mouse lung, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 127-226 of human PSMD10 (NP_002805.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EGGA NPDA KDHY EATA MHRA AAKG NLKM IHIL LYYK ASTN IQDT EGNT PLHL ACDE ERVE EAKL LVSQ GASI YIEN KEEK TPLQ VAKG GLGL ILKR MVEG
Gene ID:	5716
Uniprot:	O75832
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	16kDa/24kDa
Observed MW:	25kDa

Synonyms:

PSMD10, dJ889N15.2, p28, p28(GANK)

Background:

This gene encodes a subunit of the PA700/19S complex, which is the regulatory component of the 26S proteasome. The 26S proteosome complex is required for ubiquitin-dependent protein degradation. This protein is a non-ATPase subunit that may be involved in protein-protein interactions. Aberrant expression of this gene may paly a role in tumorigenesis. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.

UniProt Protein Function:	PSMD10: Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Proteasome complex; Protease Chromosomal Location of Human Ortholog: Xq22.3 Cellular Component: proteasome complex; nucleoplasm; intermediate filament cytoskeleton; proteasome regulatory particle; cytoplasm; nucleus; cytosol Molecular Function:protein binding; transcription factor binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of cyclin-dependent protein kinase activity; protein polyubiquitination; viral reproduction; apoptosis; negative regulation of transcription from RNA polymerase II promoter; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; negative regulation of DNA damage response, signal transduction by p53 class mediator; antigen processing and presentation of peptide antigen via MHC class I; cytoplasmic sequestering of NF-kappaB; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; negative regulation of MAPKKK cascade; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; positive regulation of cell growth; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of protein ubiquitination; inhibition of NF-kappaB transcription factor; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis
NCBI Summary:	This gene encodes a subunit of the PA700/19S complex, which is the regulatory component of the 26S proteasome. The 26S proteosome complex is required for ubiquitin-dependent protein degradation. This protein is a non-ATPase subunit that may be involved in protein-protein interactions. Aberrant expression of this gene may paly a role in tumorigenesis. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.[provided by RefSeq, Mar 2011]
UniProt Code:	O75832
NCBI GenInfo Identifier:	20532206
NCBI Gene ID:	5716
NCBI Accession:	O75832.1
UniProt Related Accession:	O75832
Molecular Weight:	16kDa; 24kDa
NCBI Full Name:	26S proteasome non-ATPase regulatory subunit 10
NCBI Synonym Full Names:	proteasome 26S subunit, non-ATPase 10
NCBI Official Symbol:	PSMD10
NCBI Official Synonym Symbols:	p28; p28(GANK); dJ889N15.2
NCBI Protein Information:	26S proteasome non-ATPase regulatory subunit 10
UniProt Protein Name:	26S proteasome non-ATPase regulatory subunit 10
UniProt Synonym Protein Names:	26S proteasome regulatory subunit p28; Gankyrin; p28(GANK)
Protein Family:	26S proteasome non-ATPase regulatory
UniProt Gene Name:	PSMD10
UniProt Entry Name:	PSD10_HUMAN

Western blot analysis of extracts of various cell lines, using PSMD10 antibody (CAB12469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

Anti-PSMD10 Antibody (CAB1949)

PSMD10 Antibody (PACO11545)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)