Anti-PSMC5 Antibody (CAB20969)

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SKU:
CAB20969
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Plant - Arabidopsis
Applications:
WB
Isotype:
IgG
Host Species:
Rabbit

Description

Antibody Name:Anti-PSMC5 Antibody (CAB20969)
Antibody SKU:CAB20969
Antibody Size:50µL, 100µL
Application:Western blotting, Immunofluorescence
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of Human PSMC5.
Application:Western blotting, Immunofluorescence
Recommended Dilution:WB 1:500 - 1:2000
IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, SKOV3, MOLT-4, Mouse liver, Mouse brain, Mouse heart, Rat testis, Rat brain
Immunogen:Recombinant protein of Human PSMC5.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Cellular Location:Cytoplasm, Nucleus
Calculated MW:44kDa/45kDa
Observed MW:46KDa
Synonyms:PSMC5, S8, SUG-1, SUG1, TBP10, TRIP1, p45, p45/SUG, ATPase 5
Background:The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. In addition to participation in proteasome functions, this subunit may participate in transcriptional regulation since it has been shown to interact with the thyroid hormone receptor and retinoid X receptor-alpha. Two transcript variants encoding different isoforms have been found for this gene.
Immunofluorescence analysis of U2OS cells using PSMC5 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of U2OS cells using PSMC5 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using PSMC5 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of NIH/3T3 cells using PSMC5 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A-549 cells using PSMC5 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of A-549 cells using PSMC5 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using PSMC5 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of HeLa cells using PSMC5 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using PSMC5 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Western blot analysis of extracts of various cell lines, using PSMC5 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
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