Anti-PSMC5 Antibody (CAB20969)
- SKU:
- CAB20969
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Plant - Arabidopsis
- Applications:
- WB
- Isotype:
- IgG
- Host Species:
- Rabbit
Description
| Antibody Name: | Anti-PSMC5 Antibody (CAB20969) |
| Antibody SKU: | CAB20969 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunofluorescence |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of Human PSMC5. |
| Application: | Western blotting, Immunofluorescence |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, SKOV3, MOLT-4, Mouse liver, Mouse brain, Mouse heart, Rat testis, Rat brain |
| Immunogen: | Recombinant protein of Human PSMC5. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 44kDa/45kDa |
| Observed MW: | 46KDa |
| Synonyms: | PSMC5, S8, SUG-1, SUG1, TBP10, TRIP1, p45, p45/SUG, ATPase 5 |
| Background: | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. In addition to participation in proteasome functions, this subunit may participate in transcriptional regulation since it has been shown to interact with the thyroid hormone receptor and retinoid X receptor-alpha. Two transcript variants encoding different isoforms have been found for this gene. |
 | Immunofluorescence analysis of U2OS cells using PSMC5 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using PSMC5 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of A-549 cells using PSMC5 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using PSMC5 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining. |
 | Western blot analysis of extracts of various cell lines, using PSMC5 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
Related Products
Anti-RANBP3 Antibody (CAB0092)
OverviewProduct Name:RANBP3 Rabbit mAbProduct Code:CAB0092Size:20uL, 50uL, 100uLSynonyms:RANBP3, ran-binding protein 3
Anti-QK1 Antibody (CAB0193)
OverviewProduct Name:QK1 Rabbit mAbProduct Code:CAB0193Size:20uL, 50uL, 100uLSynonyms:QKI, Hqk, QK, QK1, QK3, hqkI, protein quaking
Anti-DEK Antibody (CAB0315)
OverviewProduct Name:DEK Rabbit mAbProduct Code:CAB0315Size:20uL, 50uL, 100uLSynonyms:D6S231EApplications:WB,