Developmental Biology

Anti-PSMB8 Antibody (CAB7340)

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SKU:
CAB7340
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-PSMB8 Antibody
Antibody SKU:CAB7340
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 163-272 of human PSMB8 (NP_004150.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, HepG2, HL-60, NCI-H460, Mouse liver, Mouse spleen, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 163-272 of human PSMB8 (NP_004150.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LSMG SMIC GWDK KGPG LYYV DEHG TRLS GNMF STGS GNTY AYGV MDSG YRPN LSPE EAYD LGRR AIAY ATHR DSYS GGVV NMYH MKED GWVK VEST DVSD LLHQ YREA NQ
Gene ID:5696
Uniprot:P28062
Cellular Location:Cytoplasm, Nucleus
Calculated MW:29kDa/30kDa
Observed MW:23-28kDa
Synonyms:PSMB8, ALDD, D6S216, D6S216E, JMP, LMP7, NKJO, PSMB5i, RING10
Background:The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.
UniProt Protein Function:PSMB8: a proteasomal protein of the T1B peptidase family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternatively spliced isoforms have been identified; both isoforms are processed to yield the same mature subunit.
UniProt Protein Details:

Protein type:Protease; EC 3.4.25.1; Proteasome complex

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: proteasome complex; nucleoplasm; proteasome core complex; cytosol

Molecular Function:threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; fat cell differentiation; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis

Disease: Autoinflammation, Lipodystrophy, And Dermatosis Syndrome

NCBI Summary:The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq, Jul 2008]
UniProt Code:P28062
NCBI GenInfo Identifier:334302881
NCBI Gene ID:5696
NCBI Accession:P28062.3
UniProt Secondary Accession:P28062,P28063, P28064,
UniProt Related Accession:P28062
Molecular Weight:
NCBI Full Name:Proteasome subunit beta type-8
NCBI Synonym Full Names:proteasome 20S subunit beta 8
NCBI Official Symbol:PSMB8  
NCBI Official Synonym Symbols:JMP; ALDD; LMP7; NKJO; D6S216; PRAAS1; PSMB5i; RING10; D6S216E  
NCBI Protein Information:proteasome subunit beta type-8
UniProt Protein Name:Proteasome subunit beta type-8
UniProt Synonym Protein Names:Low molecular mass protein 7; Macropain subunit C13; Multicatalytic endopeptidase complex subunit C13; Proteasome component C13; Proteasome subunit beta-5i; Really interesting new gene 10 protein
UniProt Gene Name:PSMB8  
UniProt Entry Name:PSB8_HUMAN
Anti-PSMB8 Antibody (CAB7340)Western blot analysis of extracts of various cell lines, using PSMB8 antibody (CAB7340) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-PSMB8 Antibody (CAB7340)Immunohistochemistry of paraffin-embedded rat heart using PSMB8 antibody (CAB7340) at dilution of 1:100 (40x lens).
Anti-PSMB8 Antibody (CAB7340)Immunohistochemistry of paraffin-embedded human colon using PSMB8 antibody (CAB7340) at dilution of 1:100 (40x lens).
Anti-PSMB8 Antibody (CAB7340)Immunohistochemistry of paraffin-embedded human kidney using PSMB8 antibody (CAB7340) at dilution of 1:100 (40x lens).
Anti-PSMB8 Antibody (CAB7340)Immunofluorescence analysis of A549 cells using PSMB8 antibody (CAB7340). Blue: DAPI for nuclear staining.
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