Antibody Name:	Anti-PSMB7 Antibody
Antibody SKU:	CAB14771
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 44-200 of human PSMB7 (NP_002790.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, 293T, Mouse heart, Mouse testis, Mouse brain, Rat testis, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 44-200 of human PSMB7 (NP_002790.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TTIA GVVY KDGI VLGA DTRA TEGM VVAD KNCS KIHF ISPN IYCC GAGT AADT DMTT QLIS SNLE LHSL STGR LPRV VTAN RMLK QMLF RYQG YIGA ALVL GGVD VTGP HLYS IYPH GSTD KLPY VTMG SGSL AAMA VFED KFRP DMEE EEAK NLVS E
Gene ID:	5695
Uniprot:	Q99436
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	15kDa/29kDa
Observed MW:	30kDa

Synonyms:

PSMB7, Z

Background:

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. A pseudogene of this gene is located on the long arm of chromosome 14.

UniProt Protein Function:	PSMB7: a proteasomal protein of the T1B peptidase family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This catalytic subunit is downregulated by gamma interferon and proteolytic processing is required to generate a mature subunit. Not present in the immunoproteasome and is replaced by catalytic subunit 2i (proteasome beta 10 subunit).
UniProt Protein Details:	Protein type:Protease; Proteasome complex; EC 3.4.25.1 Chromosomal Location of Human Ortholog: 9q34.11-q34.12 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus; proteasome complex; proteasome core complex Molecular Function:protein binding Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; MAPKKK cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; regulation of amino acid metabolic process; regulation of mRNA stability; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway
NCBI Summary:	The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. A pseudogene of this gene is located on the long arm of chromosome 14. [provided by RefSeq, Jul 2012]
UniProt Code:	Q99436
NCBI GenInfo Identifier:	17380263
NCBI Gene ID:	5695
NCBI Accession:	Q99436.1
UniProt Secondary Accession:	Q99436,Q5TBG6, Q96AG8, Q9BWA7, B4E0P1,
UniProt Related Accession:	Q99436
Molecular Weight:	15,323 Da
NCBI Full Name:	Proteasome subunit beta type-7
NCBI Synonym Full Names:	proteasome subunit beta 7
NCBI Official Symbol:	PSMB7
NCBI Official Synonym Symbols:	Z
NCBI Protein Information:	proteasome subunit beta type-7
UniProt Protein Name:	Proteasome subunit beta type-7
UniProt Synonym Protein Names:	Macropain chain Z; Multicatalytic endopeptidase complex chain Z; Proteasome subunit Z
Protein Family:	Proteasome
UniProt Gene Name:	PSMB7
UniProt Entry Name:	PSB7_HUMAN

	Western blot analysis of extracts of various cell lines, using PSMB7 antibody (CAB14771) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of C6 cells using PSMB7 Polyclonal Antibody (CAB14771) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using PSMB7 Polyclonal Antibody (CAB14771) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.