Antibody Name:	Anti-PSMB5 Antibody
Antibody SKU:	CAB1975
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-263 of human PSMB5 (NP_002788.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, NCI-H460, Jurkat, 293T, HepG2, Mouse brain, Mouse kidney, Mouse skeletal muscle, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-263 of human PSMB5 (NP_002788.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MALA SVLE RPLP VNQR GFFG LGGR ADLL DLGP GSLS DGLS LAAP GWGV PEEP GIEM LHGT TTLA FKFR HGVI VAAD SRAT AGAY IASQ TVKK VIEI NPYL LGTM AGGA ADCS FWER LLAR QCRI YELR NKER ISVA AASK LLAN MVYQ YKGM GLSM GTMI CGWD KRGP GLYY VDSE GNRI SGAT FSVG SGSV YAYG VMDR GYSY DLEV EQAY DLAR RAIY QATY RDAY SGGA VNLY HVRE DGWI RVSS DNVA DLHE KYSG STP
Gene ID:	5693
Uniprot:	P28074
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	17kDa/21kDa/28kDa
Observed MW:	21kDa

Synonyms:

PSMB5, LMPX, MB1, X

Background:

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	PSMB5: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib. May catalyze basal processing of intracellular antigens. Plays a role in the protection against oxidative damage through the Nrf2-ARE pathway. Belongs to the peptidase T1B family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Protease; EC 3.4.25.1; Proteasome complex Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: proteasome complex; nucleoplasm; proteasome core complex; centrosome; nucleus; cytosol Molecular Function:threonine endopeptidase activity; protein binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; response to oxidative stress; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
NCBI Summary:	The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]
UniProt Code:	P28074
NCBI GenInfo Identifier:	187608890
NCBI Gene ID:	5693
NCBI Accession:	P28074.3
UniProt Secondary Accession:	P28074,Q16242, Q6PEW2, Q7Z3B5, Q86T01, Q9TNN9, B2R4N9 B4DUM9, D3DS43, E9PAV2,
UniProt Related Accession:	P28074
Molecular Weight:	Calculated MW: 17kDa, 21kDa, 28kDaObserved MW: 21kDa
NCBI Full Name:	Proteasome subunit beta type-5
NCBI Synonym Full Names:	proteasome (prosome, macropain) subunit, beta type, 5
NCBI Official Symbol:	PSMB5
NCBI Official Synonym Symbols:	X; MB1; LMPX
NCBI Protein Information:	proteasome subunit beta type-5; proteasome chain 6; proteasome subunit X; proteasome subunit MB1; macropain epsilon chain; proteasome epsilon chain; proteasome beta 5 subunit; proteasome catalytic subunit 3; proteasome subunit, beta type, 5; PSX large multifunctional protease X; multicatalytic endopeptidase complex epsilon chain
UniProt Protein Name:	Proteasome subunit beta type-5
UniProt Synonym Protein Names:	Macropain epsilon chain; Multicatalytic endopeptidase complex epsilon chain; Proteasome chain 6; Proteasome epsilon chain; Proteasome subunit MB1; Proteasome subunit X
Protein Family:	Proteasome
UniProt Gene Name:	PSMB5
UniProt Entry Name:	PSB5_HUMAN

	Western blot analysis of extracts of various cell lines, using PSMB5 antibody (CAB1975) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human stomach using PSMB5 antibody (CAB1975) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of U2OS cells using PSMB5 antibody (CAB1975). Blue: DAPI for nuclear staining.