Immunology Antibodies 2
Anti-PSMB4 Antibody (CAB5697)
- SKU:
- CAB5697
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-PSMB4 Antibody |
| Antibody SKU: | CAB5697 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human PSMB4 (NP_002787.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, A375, SKOV3, U-251MG, Mouse thymus, Mouse skeletal muscle, Mouse liver, Rat brain, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human PSMB4 (NP_002787.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEAF LGSR SGLW AGGP APGQ FYRI PSTP DSFM DPAS ALYR GPIT RTQN PMVT GTSV LGVK FEGG VVIA ADML GSYG SLAR FRNI SRIM RVNN STML GASG DYAD FQYL KQVL GQMV IDEE LLGD GHSY SPRA IHSW LTRA MYSR RSKM NPLW NTMV IGGY ADGE SFLG YVDM LGVA YEAP SLAT GYGA YLAQ PLLR EVLE KQPV LSQT EARD LVER CMRV LYYR DARS YNRF QIAT VTEK GVEI EGPL STET NWDI AHMI SGFE |
| Gene ID: | 5692 |
| Uniprot: | P28070 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 29kDa |
| Observed MW: | 22kDa |
| Synonyms: | PSMB4, HN3, HsN3, PROS-26, PROS26 |
| Background: | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. |
| UniProt Protein Function: | PSMB4: a proteasomal protein of the T1B peptidase family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. |
| UniProt Protein Details: | Protein type:Proteasome complex; Cell cycle regulation; EC 3.4.25.1; Protease; Cytoskeletal Chromosomal Location of Human Ortholog: 1q21 Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; cytosol; nucleus Molecular Function:threonine endopeptidase activity; lipopolysaccharide binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; negative regulation of inflammatory response to antigenic stimulus; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle |
| NCBI Summary: | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P28070 |
| NCBI GenInfo Identifier: | 116242733 |
| NCBI Gene ID: | 5692 |
| NCBI Accession: | P28070.4 |
| UniProt Secondary Accession: | P28070,P31148, Q5SZS5, Q6IBI4, Q969L6, B2R9L3, |
| UniProt Related Accession: | P28070 |
| Molecular Weight: | 264 |
| NCBI Full Name: | Proteasome subunit beta type-4 |
| NCBI Synonym Full Names: | proteasome (prosome, macropain) subunit, beta type, 4 |
| NCBI Official Symbol: | PSMB4 |
| NCBI Official Synonym Symbols: | HN3; HsN3; PROS26; PROS-26 |
| NCBI Protein Information: | proteasome subunit beta type-4; hsBPROS26; proteasome chain 3; macropain beta chain; proteasome beta chain; 26 kDa prosomal protein; proteasome subunit HsN3; proteasome subunit, beta type, 4; multicatalytic endopeptidase complex beta chain |
| UniProt Protein Name: | Proteasome subunit beta type-4 |
| UniProt Synonym Protein Names: | 26 kDa prosomal protein; HsBPROS26; PROS-26; Macropain beta chain; Multicatalytic endopeptidase complex beta chain; Proteasome beta chain; Proteasome chain 3; HsN3 |
| Protein Family: | Proteasome |
| UniProt Gene Name: | PSMB4 |
| UniProt Entry Name: | PSB4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PSMB4 antibody (CAB5697) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using PSMB4 antibody (CAB5697). Blue: DAPI for nuclear staining. |
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